Effect of nuclear factor-κB activation on peritoneal macrophages in rats with severe acute pancreatitis
AIM: To investigate the effect of nuclear factor-κB (NF-κB) activation from peritoneal macrophages (PMA) on the production and release of inflammatory mediators in rats with severe acute pancreatitis (SAP). METHODS: Thirty-six Sprague-Dawley rats were randomly divided into control group and SAP group. To induce severe acute pancreatitis model, 40 g/L sodium tanrecholate was injected into the pancreatic duct of rats through retrograde exposure of pancreatic duct in hepatic porta. Rats(n=6, each) were sacrificed respectively at 3, 6 or 12 h after modeling. PMAs were extracted and incubated for 24 h in a humidified 5% carbon dioxide incubator. NF-κB activation in PMA was measured with electrophoretic mobility shift assay. The levels of tumor necrosis factor-α(TNF-α) and interleukin-1β( IL-1β) in culture medium and serum were evaluated using ELISA method. RESULTS: NF-κB activation in control group was 6.26±0.79, 7.01±0.49 and 6.79±0.94 at 3, 6 and 12 h after modeling. NF-κB activation in SAP group was 11.94±1.33, 23.23±1.22 and 32.97±1.81 at each time point (P<0.01). The concentrations of TNF-α and IL-1β in culture medi-um and serum were significantly elevated in SAP group, compared with those in control group(P<0.01). CONCLUSION: NF-κB activation induces the production and the release of inflammatory mediators in PMA and SAP is related to PMA activation. The inhibition of NF-κB activation may be a promising way to treat severe acute pancreatitis.
NETL
NSTL
万方数据
维普
