Transduction of PEP-1-SOD1 fusion protein into hippocampus in mice and its time course
AIM: To explore the transductive ability of PEP-1-Cu, Zn-superoxide dismutase (PEP-1-SOD1) fusion protein into hippocampal tissue in mice and its persisting time. METHODS: Healthy adult male Kunming-mice were randomly divided into 2 groups: SOD1 treated group and PEP-1-SOD1 treated group. The mice were intraperitoneally injected with 200μg SOD1 in SOD1 treated group and 200μg PEP-1-SOD1 fusion protein in PEP-1-SOD1 treated group respectively. The animals were sacrificed at 1, 2, 4, 8 and 24 h after injection(n=5). The animals' hipp-ocampal specimens were examined with Western blot analysis, immunofluerescence staining and SOD1 activity test. RESULTS: In PEP-1-SOD1 treated group, fusion protein was expressed with Mr of 26×103 1 h after injection and reached the peak at 4 h after injection, while no protein expressed in SOD1 treated group. The hippocampal tissue treated with PEP-1-SOD1 fusion protein showed bright green fluorescent signal, but no green fluorescent signal was seen in SOD1 treated specimen. The SOD1 activity in PEP-1-SOD1 group increased tu(61.7±2.9)×103 U/g 1 h after injection, reached the peak of(82.6±4.2)×103 U/g at4 h after injection and then decreased gradually, but it was still higher than that in SOD1 treated group at 24 h after injection (P<0.01). CONCLUSION: PEP-1 mediates the transduction of nativelyactive SOD1 into hippocampal region in mice and the enzymeactivity can persist for no less than 24 h.
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万方数据
维普
