TCTP mRNA在肝再生中的表达及其生物学意义
Expression of TCTP mRNA and its biological significance in liver regeneration
朱武凌 1程海霞 1张伟丽 1张会勇 1林俊堂 1刘涌涛 1井长勤1
作者信息
- 1. 新乡医学院生命科学技术系,河南,新乡,453003
- 折叠
摘要
目的:探讨翻译控制肿瘤蛋白(TCTP)mRNA在肝再生中的表达情况和生物学意义.方法:对健康成年雄性SD大鼠进行2/3部分肝切除以建立肝再生模型,分别于肝切除术后不同时间点收集再生肝组织,在显微镜下进行有丝分裂指数评价,利用流式细胞术分析细胞周期分布变化;半定量RT-PCR法检测TCTP mRNA的表达.结果:肝细胞有丝分裂指数在术后3～12 h明显升高.细胞周期分析显示,肝切除术后1 h呈现G0/G1向s期迁移,G2/M期细胞比例在3 h呈现升高,6 h升高最为显著.TCTP mRNA的表达在肝切除术后1 h时轻微上调,在3～12 h呈显著升高,之后下降至初始水平.结论:在肝再生组织中TcTP mRNA表达呈动态变化,可能与有丝分裂和细胞增殖密切相关.
Abstract
AIM: To investigate the expression of TCTP mRNA and its biological significance in liver regeneration. METHODS: Liver regeneration model was established by a two-thirds partial hepatectomy(PH) in healthy adult male SD rats. The regenerative liver tissues were harvested at different time-points after PH. The mitotic index(MI) was evaluated under microscope and the distri-bution in G0/G1-, S- and G2/M-phase subpopulation was deter-mined by flow cytometry. The expression of TCTP mRNA was measured by semi-quantitative RT-PCR. RESULTS: After PH, the MI of hepatocytes increased significantly at 3 to 12 h. Cell cycle analysis showed a G0/G1- to S-phase transition at 1 h and an increased proportion of G2/M-phase cells at 3 h, followed by the most significant increase at 6 h. The mRNA expression of TCTP was up-regulated slightly at 1 h, markedly increased at 3 to 12 h and then declined to the original level. CONCLUSION: The dynamic changes of TCTP mRNA expression may be related to the mitosis and cell proliferation in liver regeneration.
关键词
肝再生/翻译控制肿瘤蛋白/有丝分裂/细胞周期Key words
liver regeneration/translationally controlled tumorprotein/mitosis/cell cycle引用本文复制引用
基金项目
河南省杰出青年科学基金(0512000800)
河南省高等学校杰出科研人才创新工程项目(2004kjcX017)
出版年
2009
NETL
NSTL
维普
万方数据