Effects of conditioned medium of human ADSCs on HaCaT cells
AIM: To isolate and culture adipose-derived stem cells(ADSCs) and to study the effects of cytokines secreted by ADSCs on HaCaT cells. METHODS: ADSCs were isolated from human adipose tissues and cultured for 3 d. ELISA was used to detect the secretion level of VEGF and HGF. The conditioned medium of ADSCs (ADSC-CM) was then used to culture HaCaT cells. MTT method was used to determine the proliferation and Annexin V/PI was used to examine the apoptosis rate of HaCaT cells. The cell migration was measured by in vitro wound-healing assay. RESULTS: ADSCs were isolated and cultured successfully. The amount of VEGF and HGF was (287±47) ng/L and (577±85) ng/L, respectively. The A490nm values of HaCaT cells in 30% ADSC-CM group and 50% ADSC-CM group were signifi-cantly higher than those in control group (P<0.05 or P<0.01). The apoptosis rate of HaCaT cells in experiment group was obvi-ously lower than that in control group (P<0.05). The migration distance of HaCaT cells in experiment was significantly longer than that in control group at36 and48 h(P<0.05 or P<0.01). CONCLUSION: ADSCs can secrete cytokines and ADSC-CM promotes proliferation and migration and suppresses apoptosis of HaCaT cells.
NETL
NSTL
万方数据
维普
