Experimental studies on apoptosis of A375 cells of xenografted tumors induced by nanosecond pulsed electric field and its mechanism
AIM: To investigate the effect of nanosecond steep pulse on the growth and apeptosis in A375 cells of xenografted tumors. METHODS: After establishment of 4 to 6 week-old female nude mouse models carrying human melanoma by subcuta-neous inoculationg, 20 mice were randomly divided into 2 groups: Treatment group and control group. The mice were exposed to nanosecond steep pulse(20 kV/cm, 300 ns, 1 Hz in5 min, three times every 2 d), the changes of the tumor body volume were observed and the inhibition rate was calculated. Caspase-3 was determinated by immunofluorescence and RT-PCR and the apoptesis of A375 cells was detected by transmission electron microscope. The changes of [Ca2+]i in A375 cella were observed by fluorescence spectrophotometer using Fluo-3/AM as the calcium fluorescent indicator. RESULTS: The tumor body volume in treatment group was smaller than that in control group (P<0.01) and typical morphological features of apeptotic cells were observed under transmission electron microscope. Immuno-fluorescence and RT-PCR showed that caspase-3 in treatment group increased compared with that in control group. [Ca2+]i markedly increased by treatment with nanosecond steep pulse (P<0.01). CONCLUSION: Nanosecond steep pulse induces apoptosis of transplanted tumors.
nanosecond pulsed electric fildA375 cellsapop-tosiscaspase-3concentration of Ca2+
NETL
NSTL
万方数据
维普
