Effects of Qufengtongluo Recipe on α-SMA in rats with mesangial proliferative glomerulonephritis
AIM: To study the effect and mechanism of Qufong-tongluo(QFTL) Recipe in treating rats with mesangial prolifera-tive glomerulonephritis (MsPGN) so as to provide some theoretic basis for the clinical application of Qufengtongluo Recipe. METHODS: A total of 48 rats were randomly divided into 4 groups(n=12) : Blank, model and two treatment groups(QFTL and Monopril, respectively ). MsPGN models were made by immunization in the 3 groups except in blank group. Mesangial cells(GMC) and extracelhiar matrix ( ECM ) were observed with light microscope in each group. The expression of α-SMA was observed with the immunohistochemical staining and the grey of immunohistochemical staining was analyzed with the image analysis system. RESULTS: The grey of α-SMA in glomerulus and tubules significantly decreased (P<0.05) in the model group. In the 2 treatment groups, the grey of α-SMA was enhanced significantly compared with that in model group (P<0.05), while no statistics significance was observed compared with that in blank group. No statistics significance was observed between the 2 treatment groups. In the QFTL group, the morphological changes in renal tissues showed that mesangial cells proliferated lightly and the regions of mesangial matrix broadened lightly, with no extruded phenomenon in renal tubule cave. These results were much better than those in model group. CONCLUSION: QFTL Recipe refrains the expression of α-SMA and influences the remolding of GMC. This recipe reduces pathological lesions of nephridial tissues in MsPGN rats and delays the process of glomerular sclerosis.
Qu Feng Tong Luo Recipeglomerulonephritis,membranousα-SMA
NETL
NSTL
万方数据
维普
