A new method of detecting chicken chorioallantoic membrane angiogenesis
AIM: To establish a new method to detect chicken chorioallantoic membrane (CAM) angiogenesis. METHODS: Developing CAM was separated and homogenized. The high potassium ferrocyanide solution was added to CAM homogenates and the absorbanco was detected by ultraviolet spectrophotometer. The hemoglobin content of CAM homogenates was obtained by reference standards. RESULTS: The hemoglobin content of CAM being developed for 7 and 9 d and the inhibitory effects of medium static magnetic field on the hemoglobin content of CAM were detected. The results obtained by detection of the hemoglobin content of CAM were consistent with those by stereo microscope. CONCLUSION: The high potassium ferricyanide method is a practical and quantitative approach to detect the CAM angio-genesis.
NETL
NSTL
万方数据
维普
