脂氧素对脂多糖诱导巨噬细胞炎症相关因子的影响及机制
Effects and mechanism of Lipoxin on LPS-induced inflammatory mediators in RAW264.7 macrophages
代先坤 1万敬员 1张力 2龚霞 3倪安红 4周岐新1
作者信息
- 1. 重庆医科大学药理教研室,重庆,400016
- 2. 重庆医科大学病理生理教研室,重庆,400016
- 3. 重庆医科大学人体解剖教研室,重庆,400016
- 4. 重庆医科大学华中科技大学附属梨园医院皮肤科,湖北,武汉,430030
- 折叠
摘要
目的:研究脂氧素(lipoxin A4)对脂多糖(lipopo-lysaccharide,LPS)诱导的小鼠巨噬细胞株RAW264.7中相关炎症因子如肿瘤坏死因子(TNF-α)、环加氧酶-2(COX-2)、前列腺素E2(PGE2)、血红素氧化酶-1(HO-1)和白介素-10(IL-10)的影响,并进一步探讨其内在分子机制.方法:以1mg/L LPS刺激体外培养的RAW264.7细胞作为炎症模型,分别用脂氧素A4或脂氧素A4和ZnPPIX干预LPS刺激的RAW264.7细胞24 h后,收集培养上清并提取细胞总蛋白,酶联免疫法测定上清中TNF-α,IL-10和PGE2浓度,免疫印迹法检测细胞COX-2和HO-1的蛋白表达量,分光光度计分析HO-1的活性.结果:1 mg/L LPS明显诱导TNF-α,COX-2和PGE2的生成,也适度增加IL-10及HO-1的产生;脂氧素A4可抑制LPS诱导的TNF-α(P<0.01 vs LPS组),COX-2和PGE2(P<0.01 vs LPS组)的牛成,却进一步增加IL-10(P<0.01 vs LPS组)和HO-1表达量和活性(P<0.01 vs LPS组);ZnPPIX可减弱脂氧素A4对LPS诱导TNF-α(P<0.05 vs LPS+脂氧素A4组),COX-2和PGE2(P<0.05 vs LPS+脂氧素A4组)的生成抑制作用,同时也下调IL-10的生成量.结论:脂氧素A4可抑制LPS诱导的巨噬细胞中致炎介质TNF-α,COX-2及PGE2的生成,同时也促进IL-10的产生,这种效应在一定程度上是通过上调HO-1表达和活性实现.
Abstract
AIM: To investigate the effects of lipoxin A4 on lipopolysaccharide (LPS)-induced inflammatory mediators in RAW264.7 macrophages and its underlying molecular mechanism. METHODS: RAW264. 7 cells were stimulated with 1 mg/L LPS to induce inflammatory response and then lipoxin A4 at 100 ng/mL was administrated. ZnPPIX was added to RAW264.7 cells by cotreatment of LPS and lipoxin A4. The concentrations of TNF-α, IL-10 and PGE2 in the cell supernatants were measured by ELISA. COX-2 and HO-1 proteins were detected by Western blotting and HO-1 activity was analyzed by spectrophotometer. RESULTS: Compared with that in LPS group, lipoxin A4 significantly inhibi-ted LPS-induced COX-2 protein expression and levels of TNF-α and PGE2 in RAW264.7 cells (both, P<0.01) and this effect was accompanied by a parallel increase in HO-1 and IL-10 (P<0.01). Treatment of ZnPPIX partially abolished the suppressive effects of lipoxin A4 on COX-2 protein expression and the levels of TNF-α and PGE2 in RAW264.7 cells induced by LPS, compared with those in LPS + Lipoxin A4 group ( both, P<0.05). CONCLUSION: Lipoxin A4 inhibits LPS-induced pro-inflammatory mediators, including COX-2, PGE2 and TNF-α, possibly by mediation through HO-1 pathway.
关键词
脂氧素类/脂多糖类/血红素氧化酶-1/前列腺素内过氧化物合酶Key words
lipoxins/lipopolysaccharides/heme oxygenase-1/prostaglandin-endoperoxide synthase引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据