Influence of oxidative stress on expression of apoptosis-related gene Bax and Bcl-2 in severely scalded rats at plateau
AIM: To explore the influence of oxidative stress on the expression of apoptosis-related gene Bax and Bcl-2 in severely scalded rats with delayed resuscitation at plateau. METHODS: A total of 132 Wistar rats were used as experimental models and were randomly divided into Lanzhou group and high altitude group. Lanzhou group (altitude: 1517 m) consisted of immediate fluid resuscitation group (LIFR), delayed fluid resuscitation group (LDFR) and control group (LCG). The rats were subjected to 30% TBSA scald bum on the back and were given saline resus-citation immediately or 6 h later as delayed resuscitation. The rats in high altitude group (altitude: 3840 m) were divided in the same way as Lanzhou group but the groups were named respectively as HIFR, HDFR and HCG groups. The changes of the contents of intestinal malonaldehyde (MDA) and total hydro-sulfide (TSH) were determined to observe the oxidative stress of entereeyte after burn. The expressions of Bax and Bcl-2 were detected by immunohistochemistry method. RESULTS: The levels of MDA increased and TSH decreased significantly in high altitude group than those in Lanzhou group. More Bax positive enterecytes were observed in high altitude group compared with those in Lanzhou group. The positive expression of Bax in HDFR was higher than that in LDFR. A positive relationship existed between the levels of MDA and the Bax expression of enterocytess (IFR,r=0.939, P<0.01; DFR,r=0.938, P<0.01). A negative relationship existed between the levels of TSH and the Bax expression of enterocytes (IFR, r=0.949, P<0.01 ; DFR, r=0.934, P<0.01). Bcl-2 gene was not observed in high altitude groups or 6 h and 12 h postburn in Lanzhou groups. A weak expression of Bcl-2 was observed at other time point in Lanzhou groups. CONCLUSION: Serious oxidative stress and Bax expression of enterocytes may be causes of remarkable apopto-sis of enterocytes after burn and delayed resuscitation at plateau.
NETL
NSTL
万方数据
维普
