Changes of time course of RANTES and CCR5 following myocardial infarction in rats
AIM: To investigate the changes of time course of regulated upon activation normal-cell expressed and secreted (RANTES) and its receptor chemokine (C-C motif) receptor 5 (CCR5) after myocardial infarction (MI) in rats. METHODS: The MI model was induced by ligetion of anterior descending coronary artery in SD rats. Heart function was tested 1,2, 4 and 8 weeks after surgery and the expressions of RANTES and CCR5 at mRNA and protein levels in the myocardium were detected respectively by real time RT-PCR and Western blot. RESULTS: Heart function test indicated that the MI model was successfully induced. The expressions of RANTES and CCR5 mRNA and protein increased 1 week after MI, reached the peak level in 4 weeks and were down-regulated 8 weeks post MI. Changes at protein level showed a similar pattern with those at mRNA level. CONCLUSION: As an inflammatory regulator and chemokine, RANTES can modulate inflammatory response after MI, thus contributing to the myocardial reparation after MI. Preventing the chemotaxis of RANIES and CCR5 on T cell in different stages after MI may reduce complications, ameliorate prognosis and increase the long-term survival rate.
NETL
NSTL
万方数据
维普
