Effects of total ginsenoside combined with berberine on cardiac myosin heavy chain in experimental rats with chronic congestive heart failure
AIM: To study the effects of total ginsenoside(GS) combined with berberine (Ber)on the expression of cardiac myosin heavy chain in cardiac cells of chronic congestive heart failure rats. METHODS: Animal models of heart failure were induced by subcutaneous injection of a large dose of ISO (80 mg/kg) for 12 d and were randomly divided into model group, GS + Ber group, captopril group and shenmai injection group. Some other rats served as control group. GS + Ber group were treated with total ginsenoside and berberine (each 20 mg/kg; once per day) by gavage, captopril group were given captopril (45 mg/kg; once per day) by gavage, shenmai injection group were injected intraperitoneally with shenmai injection (5 mL/kg; once per day) and model group and control group were given distilled water by gavage. After 4 weeks, hemodynamic indexes and cardiac weight index were examined and α-MHC and β-MHC mRNA expressions were detected by real-time quantitative PCR. RESULTS: LVSP, + dp/dtmax, -dp/dtmax and α-MHC mRNA expressions were lower and LVEDP, cardiac weight index and β-MHC mRNA expressions were significantly higher in control group than those in other groups(P<0.01). Compared with those in model group, LVSP, + dp/drmax,-dp/dtmax, and α-MHC mRNA expressions were higher and LVEDP, cardiac weight index and β-MHC mR-NA expressions were significantly lower in GS + Ber group and captopril group(P<0.01 or P<0.05). CONCLUSION: Total ginsenoside combined with berberine can increase α-MHC mRNA expression but decrease β-MHC mRNA expression in cardiac cells of chronic congestive heart failure rats, which may be one of the major mechanisms of improving heart function and ventricular re-modeling.
ginsenosidesberberineheart failure, congestivehemodynamic phenomenacardiac myosin heavy chain
NETL
NSTL
万方数据
维普
