2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖诱导人结肠癌SW1116凋亡的作用
Experimental study on apoptosis of human colon carcinoma cell line SW1116 induced by 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-Glucose
寇炜 1吴静 2赵晋 1兰咏梅 1窦春江 1王爱勤3
作者信息
- 1. 西北民族大学医学院技能教研室,甘肃,兰州,730030
- 2. 北京大学第九临床医院消化科,北京,100038
- 3. 中国科学院兰州化学物理研究所,甘肃,兰州,730000
- 折叠
摘要
目的:研究D-葡萄糖衍生物2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖(COPADG)对人结肠癌SW1116细胞生长增殖的影响,进而探讨COPADG诱导SW1116细胞凋亡的作用.方法:用不同浓度的COPADG处理SW1116,采用MTT法测定其对SW1116细胞生长增殖的抑制作用,通过DNA琼脂糖凝胶电泳、流式细胞术观察其对SW1116细胞诱导凋亡的效应.结果:COPADG能抑制SW1116细胞的生长增殖,并呈一定的浓度、时间依赖性;COPADG能诱导结肠癌SW1116细胞凋亡;DNA琼脂糖凝胶电泳呈现典型的DNA梯形条带图谱;流式细胞仪检测出现典型的亚二倍体凋亡峰.结论:COPADG在体外可显著诱导结肠癌SW1116细胞凋亡.
Abstract
AIM: To investigate the effect of 2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-Glucose (COPADG) on human colon carcinoma cell line SW1116 and to study the function of the COPADG in inducing cell line SW1116 apoptosis. METHODS: SW1116 cells were treated with the COPADG at various concen-trations. WIT assay was used to examine the growth and prolifera-tion of SW1116 cells after treatment with the COPADG. Agarose gel electrophoresis of DNA fragmentation and flow cytometry were used to analyze the cell line SW1116 apoptosis. RESULTS: COPADG significantly inhibited the growth and proliferation of cell line SW1116 in a dose- and time-dependent manner. Agarose gel electrophoresis analysis revealed DNA ladder and atypical sub-diploid peak was observed by flow cytometry. CONCLUSION: COPADG may inhibit SW1116 cell growth and proliferation through inducing apoptosis.
关键词
2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖/细胞凋亡/SW1116细胞Key words
2-[(3-carboxy-1-oxoprogy1) amino ]-2-deoxy-d-glucose/apoptosis/SW1116 cells引用本文复制引用
基金项目
西北民族大学中青年科研项目(D2004-021)
出版年
2009
NETL
NSTL
维普
万方数据