摘要
目的:研究TGF-β1在羟基磷灰石多孔层修饰镍钛合金修复骨缺损过程中的表达和意义,为羟基磷灰石多孔层修饰镍钛合金作为生物医学材料在临床上广泛应用提供理论依据.方法:选取健康成年兔24只,随机分为实验组和对照组,选取左侧股骨下1/3处为种植区,其中实验组植入羟基磷灰石多孔层修饰镍钛合金,对照组植入镍钛合金,通过大体观察、HE染色观察术后1,2,4,8 wk骨组织局部组织反应、骨缺损的修复情况以及免疫组织化学染色测定TGF-β1的表达.结果:大体观察、常规HE染色显示实验组1,2,4,8 wk骨痂生长、骨缺损修复情况明显优于对照组;免疫组织化学染色显示实验组于1,2,4,8 wk TGF-β1表达明显高于对照组,且具有统计学差异(P<0.05).结论:羟基磷灰石多孔层修饰镍钛合金能够刺激TGF-β1的表达,促进骨缺损修复;羟基磷灰石多孔层修饰镍钛合金与骨组织具有很好的相容性.
Abstract
AIM: To study the expression and significance of TGF-β1 in the hydroxyapatite porous layer modified nickel-titanium alloy used to repair bone defects and to provide a theoretical basis for wide clinical applications of hydroxyapatite porous layer modi-fied nickel-titanium alloy. METHODS: Twenty-four rabbits were randomly divided into experimental group and control group. Hydroxyapatite porous layer modified nickel-titanium alloy was implanted into the distal part of left femur in 12 rabbits in experi-mental group, while nickel-titanium alloy was implanted in control group. The animals were killed in 1st, 2nd, 4th and 8th week after implantation, and 3 rabbits in each group were killed at each time point. Bone tissue response to hydroxyapatite porous layer modified nickel-titanium alloy and repair of bone defects were observed. The tissue sections and expression of TGF-β1 were observed by immunohistechemical staining method with light microscopy. RESULTS: Conventional HE staining, callus growth and fracture healing in experimental group were better than those in control group in 1st, 2nd, 4th and 8th week. TGF-β1 percentage of positive cells in experimental group was significantly higher than that in control group in 1st, 2nd and 4th week, with significant difference between the 2 groups (P<0.05). CONCLUSION: Hydroxyapotite porous layer modified nickel-titanium alloy stimu-lates the expression of TGF-β1 and promotes the repair of bone defects. Hydroxyapatite porous layer modified Nickel-Titanium alloy has excellent histecompatibility with bone.
基金项目
兰州大学交叉学科青年创新研究基金(lzu200522)
NETL
NSTL
维普
万方数据