Expression and significance of TGF-β1 in hydroxyapatite porous layer modified nickel-titanium alloy used to repair bone defects
AIM: To study the expression and significance of TGF-β1 in the hydroxyapatite porous layer modified nickel-titanium alloy used to repair bone defects and to provide a theoretical basis for wide clinical applications of hydroxyapatite porous layer modi-fied nickel-titanium alloy. METHODS: Twenty-four rabbits were randomly divided into experimental group and control group. Hydroxyapatite porous layer modified nickel-titanium alloy was implanted into the distal part of left femur in 12 rabbits in experi-mental group, while nickel-titanium alloy was implanted in control group. The animals were killed in 1st, 2nd, 4th and 8th week after implantation, and 3 rabbits in each group were killed at each time point. Bone tissue response to hydroxyapatite porous layer modified nickel-titanium alloy and repair of bone defects were observed. The tissue sections and expression of TGF-β1 were observed by immunohistechemical staining method with light microscopy. RESULTS: Conventional HE staining, callus growth and fracture healing in experimental group were better than those in control group in 1st, 2nd, 4th and 8th week. TGF-β1 percentage of positive cells in experimental group was significantly higher than that in control group in 1st, 2nd and 4th week, with significant difference between the 2 groups (P<0.05). CONCLUSION: Hydroxyapotite porous layer modified nickel-titanium alloy stimu-lates the expression of TGF-β1 and promotes the repair of bone defects. Hydroxyapatite porous layer modified Nickel-Titanium alloy has excellent histecompatibility with bone.
hydroxyapotitosnickel-titanium alloybone and bonesrabbits
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万方数据
维普
