Effects of methoxychlor on Calbindin-D9k gene expression in mouse uterus during implantation period
AIM: To investigate the effects of methoxychlor (MXC) on the expression of Calbindin-D9k gene in the mouse uterus during implantation period. METHODS: Pregnant and pseudopregnant mice were given daily by intragastric administra-tion of MXC at different doses of 0 (control), 100,250 and 500 mg/kg from day 1 to day 3 of pregnancy (plug=Day 0), while the mice in the control group were given intragastrically sesame oil solvent during the same period. The expression of Calbindin-D9k mRNA was measured by RT-PCR and the expression of Calbindin-D9k protein was analyzed by immunohistochemical staining and image analysis. RESULTS: After the pregnant mice were treated with MXC for 3 d, the Calbindin-Dgk mRNA expression of the pregnant mice in groups of 250 and 500 mg/kg MXC reduced significantly (P<0.05), but no significant reduction of Calbindin-D9k mRNA was observed in mice treated with 100 mg/kg MXC (P>0.05). On day 5 of pregnancy, Calbindin-D9k protein was mainly located in the luminal and glandular epithelial cells. In parallel with its mRNA levels, the protein levels of Calbindin-D9k were significantly reduced by the treatments with MXC at 250 and 500 mg/kg doses (P<0.05), whereas no significant changes were observed in the group of 100 mg/kg MXC, compared with that in the control group (P>0.05). CONCLUSION: MXC decreases the expression of Cnlbindin-D9k gene in the mouse uterus during implantation period and may interfere with embryo implantation via this pathway.
NETL
NSTL
万方数据
维普
