镧系离子荧光光谱法测定外周血DNA在肿瘤早期诊断中的应用
Application of peripheral blood DNA with Lanthanode fluorescence spectra in early diagnosis of tumors
顾晓怡 1姜藻 1何华 2王羚郦 2邓立春1
作者信息
- 1. 东南大学附属中大医院肿瘤科,江苏,南京,210009
- 2. 中国药科大学分析化学教研室,江苏,南京,210009
- 折叠
摘要
目的:探讨镧系离子荧光光谱法测定外周血DNA在肿瘤早期诊断中的应用.方法:各提取50例恶性实体瘤、良性肿瘤、骨髓异常综合征、正常人外周血DNA,应用镧系离子荧光光谱分析法检测其荧光强度.结果:恶性实体瘤、MDS、良性肿瘤、正常人外周血DNA荧光强度分别为:346±28,308±34,280±34,276±29.恶性疾病和良性疾病之间荧光强度有统计学差异(P<0.01),且恶性疾病外周血DNA荧光强度均明显高于正常人及良性肿瘤患者.荧光强度282作为诊断恶性肿瘤的临界值,则其检测灵敏度和特异度分别为80%和72%.结论:镧系离子荧光光谱法测定外周血DNA在良、恶性肿瘤早期诊断中具有重要价值,是一种安全、简便、经济的诊断方法,并且具有较高的灵敏度和特异性.
Abstract
AIM: To investigate the application of peripheral blood DNA with Lanthanode fluorescence spectra in the early diagnosis of tumors. METHODS: Peripheral blood DNA was extracted respectively in 50 patients with malignant solid neopla-sias, myelodysplastic syndrome(MDS) or benign tumors and in 50 healthy individuals (using commercial " genomic DNA extraction kit" ), and the fluorescence intensity was detected by Lanthanode fluorescence spectra analysis. RESULTS: The mean DNA fluorescence intensity of patients with malignant solid neoplasias, MDS or benign tumors, and of healthy individuals were respectively 346±28, 308±34, 280±34 and 276±29. The fluorescence intensity of filtrating malignancy was 282, and the sensitivity and the specificity were 80% and 72%. CONCLUSION: Safe and simple, economical and effective, peripheral blood DNA fluorescence spectra analysis is very sensitive and valuable for the early diagnosis of tumors.
关键词
外周血DNA/肿瘤/光谱法,荧光/镧系离子Key words
peripheral blood DNA/neoplasms/spectrometry,fluorescence/lanthanideions引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据