Application of peripheral blood DNA with Lanthanode fluorescence spectra in early diagnosis of tumors
AIM: To investigate the application of peripheral blood DNA with Lanthanode fluorescence spectra in the early diagnosis of tumors. METHODS: Peripheral blood DNA was extracted respectively in 50 patients with malignant solid neopla-sias, myelodysplastic syndrome(MDS) or benign tumors and in 50 healthy individuals (using commercial " genomic DNA extraction kit" ), and the fluorescence intensity was detected by Lanthanode fluorescence spectra analysis. RESULTS: The mean DNA fluorescence intensity of patients with malignant solid neoplasias, MDS or benign tumors, and of healthy individuals were respectively 346±28, 308±34, 280±34 and 276±29. The fluorescence intensity of filtrating malignancy was 282, and the sensitivity and the specificity were 80% and 72%. CONCLUSION: Safe and simple, economical and effective, peripheral blood DNA fluorescence spectra analysis is very sensitive and valuable for the early diagnosis of tumors.
NETL
NSTL
万方数据
维普
