首页|同型半胱氨酸对乳腺癌患者外周血单个核细胞uPA系统表达的影响

同型半胱氨酸对乳腺癌患者外周血单个核细胞uPA系统表达的影响

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
目的:探讨Hcy是否影响乳腺癌患者外周血MNC表达uPA、uPAR和PAI-1.方法:采用不同浓度Hcy对乳腺癌患者外周血MNC进行短期(4h)培养后,粉碎细胞,ELISA法检测其uPA,uPAR和PAI-1的表达.结果:Hcy诱导乳腺癌患者外周血MNC表达uPA,uPAR,而抑制MNC表达PAI-1,并且呈剂量依赖性,Hcy浓度为0,12.5,25,50,100μmol/L时,uPA分别为47.00±21.26,57.70±26.72,72.38±38.64,83.33±42.74,94.83±44.47 ng/L;uPAR分另4为1299.3±291.7,1447.2±408.8,1607.6±467.0,1731.3±521.1,1815.3±522.3 ng/L;PAI-1分别为52.5±20.7,41.6±21.9,35.4±17.9,32.6±18.3,28.7±16.2μg/L.结论:Hcy可以影响乳腺癌患者外周血MNC表达uPA,uPAR和PAI-1,可能在乳腺癌的侵袭和转移中发挥一定的作用.
Effect of homocysteine on peripheral mononuclear cell expressing uPA system in patients with breast carcinoma
AIM: To explore whether homocysteine affects the peripheral mononuclear cells (MNC) in expressing urokinase-type plasminogen activator (uPA), urokinase-type plasminogen active receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1) in patients with breast carcinoma. METHODS: The peripheral MNCs were incubated with culture liquids of different Hcy concen-trations for 4 h and then were smashed, and the levels of uPA, uPAR and PAI-1 were measured by ELISA. RESULTS: Hcy induced the expression of uPA and uPAR but inhibited the expres-sion PAI-1 in a dose-dependent manner. CONCLUSION: Hcy affects the peripheral mononuelear cells in expressing uPA, uPAR and PAI-1, and may play a role in the invasion and metastasis of the breast carcinoma.

homocysteinemonocytesurinary plasminogen acti-vatorurokinase-type plasminogen active receptorplasminogen activator inhibitor-1breast neoplasms

王宁菊、陈耀平

展开 >

宁夏医科大学附属医院肿瘤内科,宁夏,银川,750004

宁夏医科大学附属医院医学实验中心,宁夏,银川,750004

高半胱氨酸 单核细胞 尿纤溶酶原激活物 尿纤溶酶原激活物受体 纤溶酶原激活物抑制物 乳腺肿瘤

宁夏自然科学基金

NZ0064

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子：0.599
ISSN：1000-2790
年,卷(期)：2009.30(14)
  • 4