Immunological effects of eukaryotic expression plasmid psIL-12 on allergic airway inflammation in a murine model
AIM: To observe the immunological effects of eukaryotic expression plasmid paIL-12 on airway allergic inflammation in an ovalbumin-induced (OVA) murine model. METHODS: BALB/c mice were randomized into 4 groups: psIL-12 group, pcDNA3.1(+)group, PBS/Lipofectin complexment group and the healthy control group. The pathological manifestations of the lungs, the expression of IL-12 mRNA in the lung tissues, the eosinophils count, the levels of IL-4, IFN-γ in broncho-alveolar fluid (BALF) and the level of OVA-specific IgE in serum were detected. RESULTS: It was observed that large numbers of eosinophils infiltrated the lung tissues in the pathological sections of PBS/Lipofectin complexment group, significantly higher than that in psIL-12 group. The IL-12 mRNA was positively expressed in psIL-12 group, but negatively expressed in the other groups. The percentage of eosinophil (2.6±2.3 ) %, the level of IL-4 (103±53) ng/L and the level of OVA-specific IgE in BALF of psIL-12 group decreased significantly compared with those in the control group(P ＜0.05), while the level of IFN-γ ( 171± 20) ng/L increased significantly(P＜0.05) in psIL-12 group. CONCLUSION: psIL-12 exerts some therapeutic effect through modulating the balance between TH1 and TFI2 immune response by increasing the level of IFN-γand decreasing IL-4 and specific IgE levels. It can be used as a novel treatment for allergic rhinitis and asthma.
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