Analysis of dynamic changes of interleukin 1β and prostaglandin E2 in smoke inhalation injury repair model
AIM: To investigate the dynamic changes of interleukin 1 β ( IL-1β ) and prostaglandin E2 (PGE2) levels in smoke inhalation injury repair in New Zealand white rabbits. METHODS : New Zealand white rabbits (n=36) were randomly divided into normal control group, and 1,4, 7, 14 and 21 d after smoke inhalation injury groups, with 6 animals in each group. ELISA was used to measure the levels of IL-1β and PGE2. The smoke inhalation injury repair model was established and the pathologic changes of smoke inhalation on trachea and lung tissues were observed by light microscope. RESULTS: The pathologic changes showed that mainly inflammatory reaction (injury stage)was seen in 1, 4 and 7 d after injury groups and mainly repair (repair stage) was observed in 14 and 21 d after injury groups. The results of ELISA showed that IL-1β concentration was upregulated in 1 d group[(196.2±67.8) ng/L] after smoke inhalation injury, higher than that in normal group[(7.1 +0.2) ng/L, P ＜ 0.05 ]. IL-1β concentration peaked at day 7 [ ( 398.5±161.4) ng/L] and then began to decrease. IL-1 β concentration was the lowest at day 21 [ (9.3±3.7) ng/L] and no significant differ-ence was observed compared with that in normal group (P ＞0.05). PGE2 concentrations were upregulated gradually from 1 d [(4.4±1.0) μg/L] to 14 d[(120.9±61.1) μg/L] following smoke inhalation injury. PGE2 concentrations peaked at day 14 and then began to decrease, with significant difference from those in normal control group [ (4350 ±1021 ) ng/L,P ＜0.05]. PGE2 concentration was the lowest at day 21 [(6423±2812 ) ng/L], with no significant difference from that in normal group (P＞ 0.05). CONCLUSION: The dynamic changes of IL-1β and PGE2 levels in smoke inhalation injury repair may indicate the different stages ( injury stage or repair stage) of smoke inhalation injury repair process. IL-1β and PGE2 levels may play some important role in the smoke inhalation injury repair.
NETL
NSTL
万方数据
维普
