Effect of adenovirus-mediated stromal cell-derived-factor-1 alpha gene transfer on heart function in myocardial infarction of rats
AIM: To explore the effect of adenovirus-mediated stromal cell-derived-factor-1( Adv-SDF-1 ) alpha on angiogenesis and heart function of myocardial infarction in rats. METHODS: The animal model of myocardial infarction (MI) was established by occluding the rat left anterior descending arteries (LAD). 1×1010 PFU Adv-SDF-1 or 1×1010 PFU Ad-LacZ was immediately injected into the infarcted myocordium, and 200 μL cell-free PBS was injected into the infarcted region or the myocardial wall in control and sham group, respectively. Four weeks after injection, cardiac function was analyzed. Heart tissues were taken after the measurement of hemodynamics. Histological and morphometric measurement was performed by image analysis system and expressions of CD133 and CD31 were detected by immunofhorescent histochemistry. RESULTS: Significantly higher expression of Adv-SDF-1 was observed in the Adv-SDF-1 injected MI areas, which promoted CD133 and CD31 positive cells migrating into the infarcted hearts, enhanced the density of blood vessels, and contributed to the protection and regeneration of myocardium. Decreased infarct size, reduced collagen contents and thicker left ventricle wall were observed in Adv-SDF-1 group. These changes of structure in the infarcted hearts improved the systolic and diastolic function of the heart. CONCLUSION: The high expression of SDF-1 improves cardiac structure and function of MI through angiogenesis.
NETL
NSTL
万方数据
维普
