复方苦参方剂对大肠癌LoVo细胞体外增殖凋亡的影响
Effect of proliferation and apoptosis on LoVo cells by matrine injections in vitro
徐月 1秦剑 2周远大 3何海霞3
作者信息
- 1. 重庆市中医研究院肛肠外科,重庆,400013
- 2. 重庆市中药研究院化学研究室,重庆,400065
- 3. 重庆医科大学附属第一医院临床药理学研究室,基础医学院生物工程研究室,重庆,400016
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摘要
目的:探讨复方苦参方剂对大肠癌LoVo细胞体外增殖及凋亡的影响.方法:台盼蓝染色法和MTT法测定中药对LoVo细胞抑制作用与时间和剂量的关系,用荧光显微镜、流式细胞仪对细胞凋亡进行检测.结果:复方苦参方剂可抑制Lovo细胞的生长,实验范围内其抑制作用呈时间与剂量依赖关系.经中药作用48 h后,LoVo细胞可检测细胞凋亡峰.中药组凋亡率为(7.3±1.5)%,与对照组(2.1±0.5)%相比有明显升高(P＜0.05).结论:复方苦参方剂具有一定的抑制肿瘤细胞增殖、诱导凋亡的作用.
Abstract
AIM: To investigate the effect of proliferation and apoptosis on LoVo cells by matrine injections in vitro. METHODS: The inhibition of Matrine injections on Lovo cells was detected by trypanblau staining and MTT. Fluorescence microscopy and flow cytometry were applied to study cell apoptosis of LoVo cells. RESULTS: Matrine infections inhibited the proliferation of LoVo cells in a dose-dependent manner. After treated by matrine, cell apoptosis peak was observed by flow cytometry. The apoptosis rate was(7.3±1.5)% in therapeutic group, higher than that in con-trol group (2.1±0.5) %. CONCLUSION: Matrine injection exerts some anticancer effect, possibly by inhibiting the cell prolif-eration and inducing the apeptosis.
关键词
结直肠肿瘤/苦参/增殖/凋亡Key words
colorectal neoplasms carcinoma/matrine/proliferation/apoptosis引用本文复制引用
基金项目
重庆市自然科学基金(CSTC20065030)
出版年
2009
NETL
NSTL
维普
万方数据