Effect of CK20 mRNA on hematogenous micrometastasis in laparoscopic radical resection
AIM: To investigate the effect of CK20 mRNA on the hematogenous micrometastasis in laparoscopic radical resection. METHODS: Fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect the preoperative and postoperative CK20 mRNA in the peripheral blood in 55 colorectal cancer patients treated by different surgical approach (experiment group), and 20 healthy adults were included in control group. The relationship between serum CK20 mRNA and TNM stages as well as tumor differentiation was studied and the CK20 mRNA expression of 24 colorectal cancer patients treated with laparoscopic radical surgery was compared with that of 31 patients treated by traditional laparotomy. RESULTS: The CK20 mRNA expression in control group was negative. The CK20 mRNA expression in patients with low differentiation cancers was significantly higher than that in patients with high/moderate differentiation cancers (0.75 vs 0.23, P ＜0.05). The CK20 mRNA expression of patients at phase Ⅲ significantly higher than that in patients at phase Ⅰ/Ⅱ ( P＜0.05 ). No significant difference was found between preoperative and postoperative positive rate of CK20 mRNA in patients treated by laparoscopic radical resection and no significant difference was found between the laparoscopic and open radical resection of colorectal cancer preoperatively or postoperatively. CONCLUSION: Serum CK20 mRNA in the peripheral venous blood is related to the TNM staging and tumor differentiation. Compared with traditional laparotomy, laparscopic radical resection of eolorectal cancer will not increase the positive rate of CK20 mRNA in the peripheral blood and the risk of hematogenous micrometastasis of tumor cells.
NETL
NSTL
万方数据
维普
