Correlation between expression of TLR7 mRNA in peripheral blood monouclear cells of chronic hepatitis B patients and IL-12, HBV-DNA load
AIM: To investigate the correlation between the expression of TLR7 mRNA in peripheral blood monouclear cells (PBMCs) of chronic hepatitis B patients and IL-12 and HBVDNA load and to explore the role of TLR7 in anti-HBV-DNA. METHODS: TLR7 mRNA expression was examined by real-time quantitative PCR in 38 patients with different HBV-DNA loads and 12 healthy controls, and the patients were divided into 3 groups based on low, medium or high viral load. HBV-DNA was examined by real-time quantitative PCR and the level of serum interleukin-12(IL-12) was examined by ELISA. The difference of mean between groups was studied by variance analysis and the correlation between HBV-DNA load and TLR7 expression was analyzed by Spearman rank correlation. RESULTS: The results demonstrated that the expression of TLR7 was significantly lower in HBV infection groups than that in control groups, with statisti-cally significant difference (P＜0.01). There was statistical difference in TLR7 mRNA expression among the 3 groups of chronic hepatitis B patients, and the expression of TLR7 was negatively correlated with HBVDNA load. The level of serum IL12 was significantly higher in HBV infection groups than that in controls. There was no statistical difference between the groups of low and medium virus load (P＞0.05 ), while the difference in the difference was statistically significant between other groups (P＜0.01). IL-12 was negatively correlated with HBV-DNA load (r=-0.075, P＜0.01). CONCLUSION: TLR7 mRNA expression is down-regulated in PBMCs of HBV-infected patients. Both TLR7 and IL-12 play some important roles in clearing out virus and the down-regnlation of them may relate to the chronic mechanism of hepatitis B.
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