Application of different recombinant human hematopoietic growth factors in chromosomal analysis in myeloid leukemia
AIM: To explore the potential roles of different recombinant human hematopoietic growth factors in chromosomal formation in myeloid leukemia and their applicative values. METHODS: Bone borrow simples of 22 patients with myeloid leukemia were cultured respectively with interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (GM-CSF) for 24 h and chrolnosomal formations were observed by G-binding staining. RESULTS: In the 7 patients with acute myeloid leukemia (AML), IL-3 stimulated chromosomal formation was found in 4 patients, that of GM-CSF stimulation in 3 patients and that of G-CSF-stimulation in 2 patients, and the ratio of abnormal karyotype was 57.1% (4/7). In the 15 patients with chronic myeloid leukemia(CML), the stimulatory function was found in 4 patients by IL-3, in 9 patients by GM-CSF and in 8 patients by G-CSF, and the ratio of abnormal karyotype was 100%. CONCLUSION: The effect of different hematopoietic growth factors varies significantly on the chromosomal formation in myeloid leukemia. IL-3 has stronger effects on AML while GM-CSF or G-CSF has stronger effects on CML. But for most patients, the chromosomal formation should be stimulated by one or more hematopoietic growth factors.
NETL
NSTL
万方数据
维普
