Comparative study on Fourier transform infrared spectroscopy of tissues at different tissue levels
AIM: To investigate the Fourier transform infrared spectroscopy (FTIR) spectrum of the same tissue at the level of the tissue, the cell and the plasma membrane. METHODS: Plasma membrane and remaining ingredients were extracted from cancer tissues and normal tissues, and then compressed into thin KBr disks under a pressure of 100 kg/cm2 for 8 min for FTIR investigation. The spectra obtained from the cell line, tissue, plasma membrane and remaining ingredients were compared. RESULTS: Compared with that in tissue FTIR spectrum, the absorb peak of protein and lipids related bands changed significantly in plasma membrane and remaining ingredients. The absorb peak intensity of nucleic acid related bands disappeared in plasma membrane but increased in the remaining ingredients. The absorb peak intensity of protein and lipids related bands increased in plasma membrane compared with that in tissue and cell line. The absorb peak intensity of protein and lipids related bands increased in the remaining ingredients, similarly as that in tissues. The relative intensity of I1460/I1400 in plasma membrane, remaining ingredients and cancer tissues was weaker than that in the normal tissues. CONCLUSION: The FTIR spectra, obtained from the same tissue but at different tissue levels, are different. The content of lipids decreases in cancer tissues but increases in the plasma membrane. The secondary sturctures of proteins in cancer tissues and plasma membrane change greatly. 1460 cm-1 and 1400 cm-1 can be used as reliable indicators in tumor diagnosis.
NETL
NSTL
万方数据
维普
