Influence of radiation of radioactive iodine-125 on DNA structure of liquid AFP
AIM: To study the relation of the radiated intensity and the DNA damage induced by Auger electron. METHODS: The Alpha-fetoprotein DNA solution radiated by different doses of Auger electron emitting from the radioactive iodine-125 was examined using the Laser Raman spectroscopy. RESULTS: The differentiation of characteristic Raman vibration band of DNA was extensive and its peak intensity reduced after the DNA was radiated by Auger electron in 24 h. The Raman peak of the functional groups of pyridine and purine bases, the ribosome, the phosphate bond and the conformation of DNA backbone became weak. However, the characteristic Raman peak of the DNA segments gradually emerged while the radioactive dose of iodine-125 increased. The pyridine and purine bases were dissociated and stacked and the peak intensity of the Raman spectra increased while the radiation on DNA became stronger. CONCLUSION: Auger electron emitting from iodine-125 may damage the conformation of DNA backbone and DNA structure. The damage extent of DNA is related with the radiated intensity.
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