Effects of astragalus mongholicus on primarily cultured hippocampal neurons and anoxic-ischemic injury
AIM: To explore the effects of astragalus mongholicus on the cultured hippocampal neurons in vitro and to study the protective mechanism of astragalus mongholicus on the anoxicischemic injury. METHODS: The dose-effect curve was illustrated by adding different concentrations of astragalus mongholicus into the culture medium of hippocampal neurons and the livability was measured by MIT test. The anoxic-ischemic hippocampal neuron model was established by abolishing serum from the culture medium and replacing O2 with N2 in glucose absent DMEM culture medium. The damage of anoxic-ischemic injury on the hippocampal neuron and the protective effects of astragalus mongholicus were evaluated by MTT test, LDH detection, and BDNF, NGF and NT-3 immunohistochemistry staining. RESULTS: Appropriate dosage of astragalus mongholicus increased the livability of the cultured hippocampal neurons in vitro and the best selective concentration was 300 g/L. Large dosage of astragalus mongholicus (exceeding 800 g/L) would induce toxic side effects on cultured neurons and resulted in a decrease in cell livability. Anoxicischemic injury could cause lipid peroxidation damage on hippocampal neurons with an obvious increase of LDH leakage. The anoxic-ischemie injury induced death of many hippocampal neurons, with an obvious down-regulation of BDNF. Astragalus mongholicus exerted some protective effect on the neurons suffering from anoxic-ischemic injury by decreasing the LDH leakage and up-regulating the expression of BDNF. CONCLUSION: Appropriate dosage of astragalus mongholicus increases the livability of cultured hippocampal neurons and protects the neurons from anoxic-ischemic injury.
NETL
NSTL
万方数据
维普
