Experimental study on inhibitive effect of celecoxib on lung cancer
AIM: To observe the inhibitive effect of celecoxib, a selective inhibitor of cyclooxygenase-2 (COX-2), in vitro and in vivo, on the proliferation of A549 cell line. METHODS: Antiproliferative effect was detected by MTT assay and flow cytometry was used to analyze the effect of celecoxib on cell cycles. Nude mice bearing lung cancer xenografts were treated with celecoxib and then the weight of implanted tumors was measured. The apoptotic cells in implanted tumors were stained by terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling (TUNEL) technique. RESULTS: Celecoxib inhibited the cell proliferation of lung cancer cells in a dose-dependent manner and IC50 was 50 μmol/L. Celecoxib induced the cycle arrest at G0/G1 stage, inhibiting the transition of G1 cells to S phrase. The average weight of tumor nodules in control group was (2.16 ± 0.98) g, while in the experimental group the average weight was (0.98 ± 0.51) g, with the inhibitive rate of 54.6% (P ＜ 0.05). Apoptosis of tumor cells showed that apoptotic index (AI) was 1.6 ± 0.6 in the control group and 9.5 ± 2.5 in the experimental group, with significant differences (P ＜ 0.05). CONCLUSION: Celecoxib may exert its suppressive effect in vitro and in vivo on the proliferation of A549 cell line by inducing cell cycle arrest at G0/G1 stage of A549 cell and apoptosis of the tumor cells.
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