首页|阶段性胚胎抗原-1,OCT-4,颗粒酶B在脂肪成体干细胞中的表达

阶段性胚胎抗原-1,OCT-4,颗粒酶B在脂肪成体干细胞中的表达

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  • NETL
  • NSTL
  • 万方数据
  • 维普
目的:探索脂肪成体干细胞中是否表达胚胎时期标志蛋白SSEA-1,OCT-4,GRB.方法:先将C57小鼠脂肪用Ⅰ型胶原酶消化,然后采用直接贴壁法,培养获得的细胞.取第3代生长状态稳定、形态均一的细胞进行成脂、成骨诱导分化,同时观察所培养的第3代细胞表达胚胎时期标志蛋白SSEA-1,OCT-4,GRB的情况.结果:通过该培养方法,可以获得稳定的细胞群体,并能成功进行成骨、成脂诱导分化;免疫荧光化学检测显示三种胚胎时期出现的特殊蛋白均有不同程度的表达.结论:脂肪来源的成体干细胞中表达胚胎时期的产物,这种特性可能与干细胞的多向分化特性有关系.
Expressions of SSEA-1, OCT-4 and GRB in adipose-derived adult stem cells
AIM: To observe the embryo symbol proteins (SSEA-1, OCT-4 and GRB) in mouse adipose-derived adult stem cells (ASCs). METHODS: ASCs were obtained from C57 mouse adipose tissues and were cultured in DMEM/F12 with 100 mL/L fetal bovine serum. The third passage of ASCs was induced to differentiate after exposure to adipocytic or osteogenic medium. The expressions of proteins (SSEA-1, OCT-4 and GRB) at the embryonic stage of the third passage of ASCs were observed. RESULTS: The cells isolated from adipose tissue of C57 mice had stable capability of proliferation. Osteoblast and adipocytes were induced from C57 mouse ASCs in vitro. SSEA-1, OCT-4 and GRB were expressed to different degrees in the third passage of ASCs. CONCLUSION: Adipose-derived adult stem cells express productions at the embryonic stage in C57 mice, which may be related to the multipotent differentiation of ASCs.

adipose-derived adult stem cellsinduced differentiationSSEA-1octamer-4granzyme B

陈强、杨在亮、孙士锦、张波、王正国、朱佩芳

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第三军医大学大坪医院野战外科研究所麻醉科,重庆,400042

第三军医大学大坪医院野战外科研究所创伤、烧伤与复合伤国家重点实验室第四研究室,重庆,400042

第三军医大学大坪医院野战外科研究所创伤中心,重庆,400042

脂肪成体干细胞 诱导分化 阶段性胚胎抗原-1 胚胎干细胞关键蛋白-4 颗粒酶B

国家重点基础研究发展规划(973)项目重庆市科学技术委员会科技攻关计划

2005CB522603CST2008BC5004

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子：0.599
ISSN：1000-2790
年,卷(期)：2009.30(16)
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