阶段性胚胎抗原-1,OCT-4,颗粒酶B在脂肪成体干细胞中的表达
Expressions of SSEA-1, OCT-4 and GRB in adipose-derived adult stem cells
陈强 1杨在亮 2孙士锦 3张波 2王正国 2朱佩芳2
作者信息
- 1. 第三军医大学大坪医院野战外科研究所麻醉科,重庆,400042
- 2. 第三军医大学大坪医院野战外科研究所创伤、烧伤与复合伤国家重点实验室第四研究室,重庆,400042
- 3. 第三军医大学大坪医院野战外科研究所创伤中心,重庆,400042
- 折叠
摘要
目的:探索脂肪成体干细胞中是否表达胚胎时期标志蛋白SSEA-1,OCT-4,GRB.方法:先将C57小鼠脂肪用Ⅰ型胶原酶消化,然后采用直接贴壁法,培养获得的细胞.取第3代生长状态稳定、形态均一的细胞进行成脂、成骨诱导分化,同时观察所培养的第3代细胞表达胚胎时期标志蛋白SSEA-1,OCT-4,GRB的情况.结果:通过该培养方法,可以获得稳定的细胞群体,并能成功进行成骨、成脂诱导分化;免疫荧光化学检测显示三种胚胎时期出现的特殊蛋白均有不同程度的表达.结论:脂肪来源的成体干细胞中表达胚胎时期的产物,这种特性可能与干细胞的多向分化特性有关系.
Abstract
AIM: To observe the embryo symbol proteins (SSEA-1, OCT-4 and GRB) in mouse adipose-derived adult stem cells (ASCs). METHODS: ASCs were obtained from C57 mouse adipose tissues and were cultured in DMEM/F12 with 100 mL/L fetal bovine serum. The third passage of ASCs was induced to differentiate after exposure to adipocytic or osteogenic medium. The expressions of proteins (SSEA-1, OCT-4 and GRB) at the embryonic stage of the third passage of ASCs were observed. RESULTS: The cells isolated from adipose tissue of C57 mice had stable capability of proliferation. Osteoblast and adipocytes were induced from C57 mouse ASCs in vitro. SSEA-1, OCT-4 and GRB were expressed to different degrees in the third passage of ASCs. CONCLUSION: Adipose-derived adult stem cells express productions at the embryonic stage in C57 mice, which may be related to the multipotent differentiation of ASCs.
关键词
脂肪成体干细胞/诱导分化/阶段性胚胎抗原-1/胚胎干细胞关键蛋白-4/颗粒酶BKey words
adipose-derived adult stem cells/induced differentiation/SSEA-1/octamer-4/granzyme B引用本文复制引用
基金项目
国家重点基础研究发展规划(973)项目(2005CB522603)
重庆市科学技术委员会科技攻关计划(CST2008BC5004)
出版年
2009
NETL
NSTL
维普
万方数据