Effects of gastrodin on free fatty acid release and antioxidant activities from isolated adipose tissue in rats
AIM: To study the effects of gastrodin on fee fatty acid (FFA) release and antioxidant activities from isolated adipose tissue in rats and to explore its mechanism. METHODS: The rats were divided into 8 groups: control group, adrenaline hydrochloride group, low, medium or high gastrodin groups (The concentrations of gastrodin were 1.0 × 10-5, 1.0 10-6, 1.0 × 10-7 mol/L, respectively), medium gastrodin plus verapamil group, medium gastrodin plus propranolol group, and medium gastrodin plus adenylyl cyclase inhibitor (SQ22, 536) group. Isolated rat adipose tissue was added to each group respectively. After incubated for 90 min, the level of FFA, total antioxidant capacity (T-AOC) and malonaldehyde (MDA) were measured. RESULTS: Compared with control group, Gastrodin dose-dependently increased FFA release, but its effect was weaker than that of Adrenaline hydrochloride. Gastrodin also raised T-AOC and reduced MAD in Adrenaline hydrochloride group and medium gastrodin group (P ＜ 0.01). However, compared with those in medium gastrodin group, the levels of FFA were significantly reduced in medium gastrodin plus verapamil group, medium gastrodin plus propranolol group and medium gastrodin plus SQ22, 536 group (P＜ 0.05). CONCLUSION: Gastrodin dose-dependently increases FFA release from isolated adipose tissue in rats. Its mechanism may partly be mediated by β-adrenoceptor, varapamil-sensitive L-Ca2+ channel and adenylyl cyclase. Gastrodin has antioxidant capacity in the isolated adipose tissue and reduces the extent of lipid peroxidation.
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万方数据
维普
