Effect of acute cold stress on levels of serum glucose and serum hormone in rats
AIM: To study the effects of acute cold stress on the body temperature, blood glucose level and serum hormone levels in rats. METHODS: Rats were subjected to a single 3 h period of cold exposure (- 15℃) and control animals were placed inside rat cages for the same duration. The body temperature and serum glucose level were determined. Fluorospectrophotometry and radio immunoassay were performed to determine the serum hormone levels. RESULTS: The rectal temperature of the rats decreased by 2.80℃, accompanied with a decrease of serum glucose level, both of which were still lower than those of the controls 30 min after the cold exposure. Levels of serum corticosterone (CORT), adrenocorticotrophic hormone (ACTH) and 5-hydroxytryptamine (5-HT) significantly increased under cold stress conditions, while norepinephrine (NE) level decreased and dopamine (DA) level remained unchanged. CONCLUSION: Cold exposure may affect the regulation of body temperature and energy metabolism, and lead to the activation of hypothalamic-pituitary-adrenal (HPA) axis.
NETL
NSTL
万方数据
维普
