Extraction of mulberry leaf polysaccharide and its effect on immune function of mice
AIM:To optimize the water extraction techniques of polysaccharide from mulberry leaves and to explore the effect of mulberry leaf polysaccharide on the immune function of mice. METHODS: The conditions influencing the water extraction of polysaccharide were analyzed and optimized using the orthogonal test and the effects of the extracts on the carbon particle clearance coefficients, macrophage phagocytosis, serum hemolysin titer (HC50), the amount of plaque forming cells (quantitative hemolysis spectrophotometrically, QHS), and functions of T-and B-lymphocytes were studied in the mouse models of poor immune function induced by peritoneal injection of cyclophosphamide. RESULTS: The optimum conditions for the water extraction of polysaccharide from mulberry leaves were: the ratio of reagent to liquid was 1:8, heating at 80℃ for 1.5 h and one-time recirculation. The extracts increased the κ and a coefficients of carbon particle clearance, the levels of HC50 and QHS (P ＜ 0.05 - 0.01), the phagocytic rate and indexes of macrophages, and T- and B-lymphocyte functions (P ＜ 0.01) in the mouse models. CONCLUSION: Our optimized extraction techniques for mulberry leaf polysaccharide are stable and feasible and the extracts enhance the immune function of mice.
mulberry leaf polysaccharideextraction techniquesimmune function
NETL
NSTL
万方数据
维普
