Changes and clinical significance of helper lymphocyte T subsets in patients with acute myocardial infarction
AIM: To investigate the differences of Th1 and Th2 cell numbers as well as the plasma levels of IFN-γ and IL-4 between patients with acute myocardial infarction (AMI) and the patients with chest pain but normal coronary arteriongraphy (control group), and to study the relationship between helper lymphocyte T involved immune-inflammatory reactions and AMI. METHODS: Density gradient centrifugation was employed to isolate mononuclear cells from AMI patients (n = 15) and patients with chest pain but normal coronary arteriongraphy (n = 15). Flow cytometric analysis was applied to detect the numbers of Th1 and Th2 and ELISA was used to analyze the plasma levels of IFN-γ and IL-4. RESULTS: The cell numbers of Th1 and plasma levels of IFN-γ were significantly higher in occlusion coronary artery compared with those in peripheral blood in AMI patients (P ＜ 0.05), but the levels of Th1 and IFN-γ were significantly higher in peripheral blood of AMI patients than those in control group (P ＜ 0.05). However, no statistically significant difference was observed between the levels of Th1 and IFN-γ in coronary artery and peripheral blood of control group. The cell numbers of Th2 and plasma levels of IL-4 were also significantly higher in occlusion coronary artery compared with those in peripheral blood in AMI patients (P ＜ 0.05), while the levels of Th2 were higher in peripheral blood of AMI patients than those in control group (P ＜ 0.05). No statistically significant difference was found between the levels of IL-4 in peripheral blood of AMI patients and those in control group, and no statistically significant difference was observed between the levels of Th2 and IL-4 in coronary artery and peripheral blood of control group. CONCLUSION: Th1 and Th2 subsets of CD4+ T lymphocytes may play important roles in immune-inflammatory reactions in AMI and atherosclerosis.
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万方数据
维普
