缺血预处理时内源性强啡肽与κ阿片受体对缺血再灌注心肌的保护作用
Protective effects of endogenous dynor-phin and kappa opioid receptor in ischemic preconditioning against myo-cardium ischemia/reperfnsion injury
叶明翔 1樊荣 2魏保真 2周京军 2胡玉珍 2王跃民 2裴建明2
作者信息
- 1. 第四军医大学口腔医学系2006级学员十队,陕西西安710033
- 2. 第四军医大学基础部生理学教研室,陕西西安710033
- 折叠
摘要
明显增高(P<0.05),但心肌mRNA水平尚无明显变化.κ阿片受体阻断剂Nor-BNI能够阻断心肌IPC对于I/R组大鼠心肌收缩功能的改善作用.结论:IPC可通过增加血浆Dyn水平激活κ阿片受体产生对心脏功能的保护作用.
Abstract
AIM: To investigate the effects of ischemic preconditioning on endogenous dynorphin content of blood plasma and mRNA of ventricular myocytes in rats and the protective effects of dynorphin and kappa opioid receptor against myocardium ischemia/ reperfusion injury. METHODS: Forty-eight rats were randomly divided into 6 groups: control group, sham group, ischemic preconditioning group (IPC), ischemia/reperfusion group (I/R), IPC + I/R group and Nor-BNI + IPC + I/R(Nor-BNI, a selective kappa opioid receptor antagonist) (n = 8, each). Radioimmunoassay was employed to measure the dynorphin content of blood plasma and polymerase chain reaction (PCR) was used to measure mRNA of ventricular myocytes. The influence of pre-administration of a selective kappa opioid receptor antagonist before IPC was also observed. RESULTS: The level of dynorphin content of blood plasma significantly increased in IPC group compared with that in 30 min sham group (P ＜ 0.05), but mRNA of ventricular myocytes showed no significant difference. Left ventricular systole pressure (LVSP) and ± dp/dtmax significantly decreased with administration of Nor-BNI (2 mg/kg) at 10 and 30 min of ischemia as well as 30 min of reperfusion (timing from 30 min of ischemia) compared with those in IPC + I/R group (P ＜ 0.05). CONCLUSION: IPC promotes the release of endogeneous dynorphin and increases the level of dynorphin content of blood plasma in rats. Blockade of kappa opioid receptor blunts the protective effects of IPC on the recovery of cardiac function.
关键词
缺血预处理/心肌/心肌再灌注损伤/κ阿片受体/Nor-BNI/血浆强啡肽水平/心肌mRNAKey words
ischemic preconditioning/myocardial reperfusion injurg/kappa opioid receptor/Nor-BNI/level of dynorphin content of blood plasm/mRNA of myocardium引用本文复制引用
基金项目
国家自然科学基金(30770802)
全军医药卫生科研基金(06MA203)
出版年
2009
NETL
NSTL
维普
万方数据