Effect of NF-κB inhibitor on no-refiow and expression of ICAM-1 and P-selectin
AIM: To investigate the effect of pyrrolidine dithiocarbamate(PDTC), an inhibitor of nuclear factor-κB (NF-κB), on the no reflow after reperfusion and the expressions of intercellular adhesion molecule-1 (ICAM-1) and P-selectin in rats with acute myocardial infarction (AMI). METHODS: Thirty-six SD rate were randomly divided into sham-operated group, control group and PDTC group. To establish the animal model of no reflow, the left anterior descending coronary arteries of rats underwent 3 h of ligation and 2 h of reperfusion. The area of no-reflow (ANR%) was determined by pathological staining and proteins and mRNA expressions of ICAM-1 and P-selectin were detected by immuno-fluorescent staining and RT-PCR. RESULTS: Compared with those in sham-operated group, the proteins and mRNA expression of ICAM-1 and P-selectin in the ischemic area significantly increased in control group(P ＜ 0.01), while those in PDTC group were significantly inhibited(P ＜ 0.05). The area of no reflow in PDTC group significantly decreased compared with that in control group(P ＜ 0.05). CONCLUSION: PDTC significantly reduces the area of no-reflow after reperfusion in rats with AMI, possibly by the suppression of ICAM-1 and P-selectin gene expressions.
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万方数据
维普
