NF-κB抑制剂对大鼠AMI再灌注后无再流及ICAM-1,P-选择素表达的影响
Effect of NF-κB inhibitor on no-refiow and expression of ICAM-1 and P-selectin
何泉 1雷寒 1柳青 1覃数 1马康华 1王曦1
作者信息
- 1. 重庆医科大学附属第一医院心内科,重庆400016
- 折叠
摘要
PDTC使缺血区ICAM-1,P-选择索蛋白及mRNA表达较对照组显著减弱(P<0.05);PDTC组无再流范围较对照组显著减小(P<0.05).结论:NF-κB抑制剂PDTC能显著减小大鼠AMI再灌注后无再流范围,其作用可能与抑制再灌注后缺血区ICAM-1及P-选择素的表达有关.
Abstract
AIM: To investigate the effect of pyrrolidine dithiocarbamate(PDTC), an inhibitor of nuclear factor-κB (NF-κB), on the no reflow after reperfusion and the expressions of intercellular adhesion molecule-1 (ICAM-1) and P-selectin in rats with acute myocardial infarction (AMI). METHODS: Thirty-six SD rate were randomly divided into sham-operated group, control group and PDTC group. To establish the animal model of no reflow, the left anterior descending coronary arteries of rats underwent 3 h of ligation and 2 h of reperfusion. The area of no-reflow (ANR%) was determined by pathological staining and proteins and mRNA expressions of ICAM-1 and P-selectin were detected by immuno-fluorescent staining and RT-PCR. RESULTS: Compared with those in sham-operated group, the proteins and mRNA expression of ICAM-1 and P-selectin in the ischemic area significantly increased in control group(P ＜ 0.01), while those in PDTC group were significantly inhibited(P ＜ 0.05). The area of no reflow in PDTC group significantly decreased compared with that in control group(P ＜ 0.05). CONCLUSION: PDTC significantly reduces the area of no-reflow after reperfusion in rats with AMI, possibly by the suppression of ICAM-1 and P-selectin gene expressions.
关键词
急性心肌梗死/无再流现象/P-选择素/细胞间粘附分子-1/核因子-κBKey words
acute myocardial infarction/no reflow phenomenon/P-selectin/ICAM-1/neuclear factor-κB引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据