Effect of ambroxol on virulence factors and expression of relative genes of Quorum-sensing system in pseudo-monas aeruginosa biofilm
AIM: To study the effect of ambroxol on the virulence factors of quorum-sensing (QS) system in pseudomonas aeruginosa biofilm(BF) and on the expression of the genes associated with virulence factors in an established biofilm model in vitro. METHOD: Mature pseudomonas aeruginosa BF was formed after 7 days' culture on plate. After the treatment of ambroxol at different concentrations, quantitative real-time RT-PCR was used to analyze the expression of virulence factors coding genes including toxA, rhlA, lasB and aprA, and ELISA was used to quantify the exotoxin A and elastase. Orcin-sulphuric acid method was used to quantify rhamnolipid and Folin-phenol colorimetry was utilized to quantify the activity of alkaline protease. RESULTS: After the treatment of ambroxol at the concentration of 3.750 g/L, the mRNA expressions of toxA, rhlA, lasB and aprA were down-regulated(P ＜ 0.05) and the contents of exotoxin A, elastase, rhamnolipid and alkaline protease decreased respectively(P ＜ 0.05). Ambroxol at the concentration of 1.875 g/L displayed the same tendency, though less obvious (P ＜ 0.05). CONCLUSION: Ambroxol down-regulates the virulence factors coding genes and the virulence factors of Quorum-sensing system in pseudomonas aeruginosa biofilm.
NETL
NSTL
万方数据
维普
