Effect of degraded amorphophallus konjac polysaccharides on experimental colitis induced by trinitro-benzene-sulfonic acid in rats
AIM: To investigate the effect of degraded amorphophallus konjac polysaccharides (DAKP) on trinitro-benzene-sulfonic acid(TNBS)-induced colitis in rate. METHODS: Sixty SD rats were randomly and equally divided into 6 groups: control, model, dexamethasone(DX) and three DAKP(50, 100, 200 mg/kg) groups. All the rats except those in control group were treated with TNBS/ethanol to induce colitis. The rats in DAKP and DX groups were treated intragastrically with DAKP(50, 100, 200 mg/kg) or DX(0.2 mg/kg). Treatments with saline, DAKP or DX started 6 h after TNBS enema, once a day for 5 consecutive days. At the 6 th day, all the rats were sacrificed by overdosed ether and peritoneal macrophage and colon tissue were obtained. Tumor necrosis factor-α(TNF-α) was assessed by ELISA. 8 cm colon were cut and weighed, and the ulcer area and colon index were measured. The isolated colonic mucosa was frozen in liquid nitrogen and MPO activity was tested. 2 cm colon was formaldehyde fixed and HE stained, and thymus and spleen were weighed. RESULTS: Compared with those in model group, DAKP(100, 200 mg/kg) significantly attenuated the colon index and colon edema, decreased the ulcer area(P ＜ 0.05). The content of TNF-α in DAKP(100, 200 mg/kg) groups was significantly lower than that in model group (P ＜ 0.05), but no significant difference was seen compared with that in DX group. CONCLUSION: DAKP has good therapeutic effects on colitis in rats, in which polysaccharide may play a vital role.
NETL
NSTL
万方数据
维普
