降解魔芋多糖对三硝基苯磺酸诱导实验性结肠炎的治疗作用
Effect of degraded amorphophallus konjac polysaccharides on experimental colitis induced by trinitro-benzene-sulfonic acid in rats
王慧 1刘莉 2杨铁虹 2孙阳 2冯娟 2李宇华 2梅其炳2
作者信息
- 1. 第四军医大学药学系药理学教研室,陕西西安710033;解放军第323医院药剂科,陕西西安710054
- 2. 第四军医大学药学系药理学教研室,陕西西安710033
- 折叠
摘要
5.0±1.0)vs(161.7 ±2.3),P<0.05].TNF-α表达DAKP 100,200mg/kg剂量组低于模型组[(215.1 ±7.2),(201.3±5.5)vs(306.9 ±8.1),P<0.05];与DX组相比较其疗效差异无显著性.结论:DAKP对TNBS诱导的大鼠结肠炎具有显著治疗作用.低分子DAKP可能是调节胃肠道功能的主要有效成分.
Abstract
AIM: To investigate the effect of degraded amorphophallus konjac polysaccharides (DAKP) on trinitro-benzene-sulfonic acid(TNBS)-induced colitis in rate. METHODS: Sixty SD rats were randomly and equally divided into 6 groups: control, model, dexamethasone(DX) and three DAKP(50, 100, 200 mg/kg) groups. All the rats except those in control group were treated with TNBS/ethanol to induce colitis. The rats in DAKP and DX groups were treated intragastrically with DAKP(50, 100, 200 mg/kg) or DX(0.2 mg/kg). Treatments with saline, DAKP or DX started 6 h after TNBS enema, once a day for 5 consecutive days. At the 6 th day, all the rats were sacrificed by overdosed ether and peritoneal macrophage and colon tissue were obtained. Tumor necrosis factor-α(TNF-α) was assessed by ELISA. 8 cm colon were cut and weighed, and the ulcer area and colon index were measured. The isolated colonic mucosa was frozen in liquid nitrogen and MPO activity was tested. 2 cm colon was formaldehyde fixed and HE stained, and thymus and spleen were weighed. RESULTS: Compared with those in model group, DAKP(100, 200 mg/kg) significantly attenuated the colon index and colon edema, decreased the ulcer area(P ＜ 0.05). The content of TNF-α in DAKP(100, 200 mg/kg) groups was significantly lower than that in model group (P ＜ 0.05), but no significant difference was seen compared with that in DX group. CONCLUSION: DAKP has good therapeutic effects on colitis in rats, in which polysaccharide may play a vital role.
关键词
降解魔芋多糖/地塞米松/溃疡性结肠炎/2,4,6-三硝基苯磺酸Key words
degraded amorphophallus konjac polysaccharides/dexamethasone/ulcerative colitis/2, 4, 6-trinitrobenzene-sulfonic acid引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据