互隔交链孢酚激活EC9706细胞DNA聚合酶β表达的信号通路初探
A preliminary study of signal trusduction of DNA polymerase β expression in EC9706 cells induced by alternariol
赵继敏 1李沛 1杨洪艳 1黄幼田 1江亚南 1赵明耀 1郑智敏 1董子明1
作者信息
- 1. 郑州大学基础医学院病理生理学教研室,河南郑州450052
- 折叠
摘要
示,与溶剂对照组和H89处理组相比较,AOH处理组,Forskolin处理组的细胞中polβ表达增高.Western Blot结果表明,AOH处理组,Forskolin处理组pol β表达分别是溶剂对照组的2.05和3.12倍(P<0.05),而H89组仅为对照组的1.32倍.结论:AOH可以激活食管癌EC9706细胞中pol β基因表达,这可能是通过PKA信号转导通路介导的.
Abstract
AIM: To investigate the expression of DNA polymerase β(pol β) induced by Alternariol(AOH) in human esophageal squamous cell carcinoma (ESCC) EC9706 cell line and to study the relation between the expression of DNA pol β and PKA signal pathway. METHODS: EC9706 cells were treated with 20 μmol/L AOH and 40 μmol/L Forskolin (PKA activator) for 16 h. The cells were pretreated with an inhibitor of PKA(H89) for 1 h and then were exposed to 20 μmol/L AOH for 16 h. The changes of pol β expression were detected by immunocytochemistry analysis and Western blotting. RESULTS: The results of immunocytochemistry indicated that the expression of pol β increased in the AOH group and Forskolin group, compared with that in control group and H89 group. Western blotting showed that the expression of pol β in AOH group and Forskolin group was respectively 2.05 times and 3.12 times higher than that in control group (P ＜ 0.05), while the expression in the H89 group was only 1.32 times higher than that in control group. CONCLUSION: AOH up-regulates DNA pol β expression in EC9706 cells, which may be mediated by the PKA signal transduction pathway.
关键词
DNA聚合酶β/互隔交链孢酚/蛋白激酶A/信号转导通路Key words
DNA polymerase β/alternariol/protein kinase A/signal transduction pathway引用本文复制引用
基金项目
国家自然科学基金(30471952)
河南省教育厅自然科学研究计划项目(2007310020)
出版年
2009
NETL
NSTL
维普
万方数据