A preliminary study of signal trusduction of DNA polymerase β expression in EC9706 cells induced by alternariol
AIM: To investigate the expression of DNA polymerase β(pol β) induced by Alternariol(AOH) in human esophageal squamous cell carcinoma (ESCC) EC9706 cell line and to study the relation between the expression of DNA pol β and PKA signal pathway. METHODS: EC9706 cells were treated with 20 μmol/L AOH and 40 μmol/L Forskolin (PKA activator) for 16 h. The cells were pretreated with an inhibitor of PKA(H89) for 1 h and then were exposed to 20 μmol/L AOH for 16 h. The changes of pol β expression were detected by immunocytochemistry analysis and Western blotting. RESULTS: The results of immunocytochemistry indicated that the expression of pol β increased in the AOH group and Forskolin group, compared with that in control group and H89 group. Western blotting showed that the expression of pol β in AOH group and Forskolin group was respectively 2.05 times and 3.12 times higher than that in control group (P ＜ 0.05), while the expression in the H89 group was only 1.32 times higher than that in control group. CONCLUSION: AOH up-regulates DNA pol β expression in EC9706 cells, which may be mediated by the PKA signal transduction pathway.
DNA polymerase βalternariolprotein kinase Asignal transduction pathway
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