Effects of sulodexide on osteoporosis in experimental diabetic rats
AIM: To study the effects of sulodexide on osteoporosis in experimental diabetic rats. METHODS: Sprague-Dawley (SD) rats were randomly divided into normal control group(C), diabetic group without treatment (D) and sulodexide treatment group(S), and a single dose of streptozotocin was abdominally injected to establish the diabetic rat models. Each animal in sulodexide treated group was additionally fed daily with sulodexide of 10 mg/(kg · d) per day for 12 weeks, while the animals in the C and D groups were given only normal water during the same period. After 12 weeks of treatment, blood glucose and body weight were measured. Bone histomorphometry and bone mineral density were analyzed, and bone mineral density(BMD) of lumbar spines was determined by dual energy X-ray absorption (DEXA). Reverse transcription-polymerase reaction (RT-PCR) was performed to detect the expression of OPG mRNA and RANKL mRNA in the sample bones. RESULTS: After 12 weeks, histomorphometry and the bone mineral density analysis showed that; osteoporosis was found in both D group and S group, and osteoporosis in S group was similar to that in D group. Compared with those in C group, mean trabecular plate thickness (MTPT) significantly decreased (P ＜ 0.01) and mean trabecular plate separation (MTPS) significantly elevated(P ＜ 0.01) in both D group and S group. Compared with those in D group, MTPT and MTPS had no obviously changes in S group. BDM results showed that: Compared with that in C group, BDM in D group and S group significantly decreased(P ＜ 0.01). BDM in S group had no significant changes compared with those in D group. RT-PCR showed that: OPG mRNA level decreased in D group and S group(P ＜ 0.05). RANKL mRNA level increased in both D group and S group (P ＜ 0.05). No significant difference was found in OPG mRNA and RANKL mRNA levels between D group and S group. CONCLUSION: Diabetic rats suffer from osteopnia and osteoporosis but sulodexide has no obvious effect on diabetic osteoporosis in diabetic rats.
diabetic ratsosteoporosissulodexideosteoprotegerinreceptor activator of NF-KB ligand
NETL
NSTL
万方数据
维普
