吗啡对心肌缺血再灌注大鼠血清TNF-α和血浆ET-1的影响
Effect of morphine on serous TNF-α and plasmatic ET-1 in acute myocardial ischemic reperfusion injury in rats
丁永刚 1张正义 1李培杰 1赵建洪 1陈天铎 1严洲 1昝香怡 1汤晓琴2
作者信息
- 1. 兰州大学第二医院急诊科,甘肃兰州730030
- 2. 兰州大学基础医学院解剖学教研室,甘肃兰州730000
- 折叠
摘要
.00),P<0.01],而B组与D组浓度差异无显著性.B组与A,C组相比较心肌梗死范围缩小,差异具有显著性[(22±5)%vs(38±6)%,(22±5)%vs(36±4)%,P<0.01];而A,C组心肌梗死面积无显著差异.结论:吗啡预处理可抑制血清TNF-α,血浆ET-1的浓度,从而保护缺血再灌注对心肌细胞的损伤.
Abstract
AIM: To observe the influence of morphine on serous TNF-α and plasmatic ET-1 concentration and to study its protective effect on acute myocardial ischemic-reperfusion (AMIR) injury in rats. METHODS: Forty SD rate were divided randomly into 4 groups: ischemic-reperfusion group (group A), morphine preconditioning group (group B), morphine and nalox-one group (group C), and normal control group (group D). The animal model of AMIR was established in rats by tying the left anterior descending branch (LAD) of rat coronary for 30 min and then untying for 4 h. The animals were then sacrificed and hearts were harvested for determination of myocardial infarct size by 2, 3, 5-triphenyltetrazolium chloride (TTC). Radioimmunoassay was conducted to detect the tumor necrosis factor alpha (TNF-α) in serum and endothelin-1 (ET-1) in plasma. RESULTS: At 4.5 h of myocardial ischemic-reperfusion (MIR), both TNF-α concentration in serum and ET-1 concentration in plasma increased significantly in Croup A and Group C compare with those in Group B (both P ＜ 0.01), but no significant difference was observed between Group B and Group D. The myocardium infarct size was markedly smaller in group B compared with that in Group A and Group C (P ＜ 0.01), but no significant difference was found between Group A and Group C. CONCLUSION: Morphine exerts protective effects on acute myocardial ischemic (AMI) by decreasing serum TNF-α and plasmatic ET-1 concentration and shrinking myocardial infarct size.
关键词
心肌缺血/心肌再灌注损伤/肿瘤坏死因子-α/内皮缩血管肽/吗啡Key words
myocardial ischemic-myocardial/reperfusion injury/TNF-α/ET-1/morphine引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据