Effect of morphine on serous TNF-α and plasmatic ET-1 in acute myocardial ischemic reperfusion injury in rats
AIM: To observe the influence of morphine on serous TNF-α and plasmatic ET-1 concentration and to study its protective effect on acute myocardial ischemic-reperfusion (AMIR) injury in rats. METHODS: Forty SD rate were divided randomly into 4 groups: ischemic-reperfusion group (group A), morphine preconditioning group (group B), morphine and nalox-one group (group C), and normal control group (group D). The animal model of AMIR was established in rats by tying the left anterior descending branch (LAD) of rat coronary for 30 min and then untying for 4 h. The animals were then sacrificed and hearts were harvested for determination of myocardial infarct size by 2, 3, 5-triphenyltetrazolium chloride (TTC). Radioimmunoassay was conducted to detect the tumor necrosis factor alpha (TNF-α) in serum and endothelin-1 (ET-1) in plasma. RESULTS: At 4.5 h of myocardial ischemic-reperfusion (MIR), both TNF-α concentration in serum and ET-1 concentration in plasma increased significantly in Croup A and Group C compare with those in Group B (both P ＜ 0.01), but no significant difference was observed between Group B and Group D. The myocardium infarct size was markedly smaller in group B compared with that in Group A and Group C (P ＜ 0.01), but no significant difference was found between Group A and Group C. CONCLUSION: Morphine exerts protective effects on acute myocardial ischemic (AMI) by decreasing serum TNF-α and plasmatic ET-1 concentration and shrinking myocardial infarct size.
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万方数据
维普
