不同种属来源真皮间充质干细胞对人皮肤成纤维细胞组织修复作用的影响
Effect of heterogeneously dermis-derived mesenchymal stem cells on human skin fibroblasts tissue repair
毛越苹 1齐庆 2谭国珍 1郭庆 1曾凡钦1
作者信息
- 1. 中山大学附属第二医院皮肤性病科,广东广州510120
- 2. 广州中医药大学附属第一医院皮肤性病科,广东广州510405
- 折叠
摘要
1含量差异具有统计学意义(F=0.699,P<0.05).结论:不同来源真皮MSCs与hsFb共培养对胶原分泌和TGF-β1表达无影响,组织修复过程中,异种属真皮MSCs的诱导是可靠的.
Abstract
AIM: To compare the tissue repair effect of mouse dermis-derived mesenchymal stem cells(mdMSCs)and human dermis-derived MSCs (hdMSCs) on normal human skin fibroblast(hsFb) and to explore the reliability of heterogeneously dermis-derived MSCs in tissue repair. METHODS: mdMSCs and hdMSCs were isolated and cultivated by tissue digested-adherent-passage in low-serum medium and the changes of hydroxyproline(Hyp) and TCF-β1 were determined in co-culture system of mdMSCs or hdMSCs and hsFb on day 1 and 4 by samples basic hydrolysis and ELISA respectively. RESULTS: No significant difference was found in the contents of Hyp between groups of different MSCs(F = 0.174) or between groups of different cell density (F = 1.304). The contents of Hyp were significantly different between day 1 and day 4 (F = 8.712, P ＜ 0.01). The level of TGF-β1 was not significantly different between groups of different MSCs(F = 0.062) or between groups of different cell density (F = 1.991). The level of TGF-β1 was significantly different between day 1 and day 4 (F = 0.699, P ＜ 0.05). CONCLUSION: Dermal MSCs of different origin (mouse or human) would not affect the levels of collagen and TGF-β1 when cultivated with hsFb. It is reliable for heterogeneously dermis-derived MSCs to promote tissue repair on hsFb.
关键词
真皮/间充质干细胞/异种动物/成纤维细胞Key words
dermis/mesenchymal stem cells/outbred strains animals/fibroblasts引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据