Effect of heterogeneously dermis-derived mesenchymal stem cells on human skin fibroblasts tissue repair
AIM: To compare the tissue repair effect of mouse dermis-derived mesenchymal stem cells(mdMSCs)and human dermis-derived MSCs (hdMSCs) on normal human skin fibroblast(hsFb) and to explore the reliability of heterogeneously dermis-derived MSCs in tissue repair. METHODS: mdMSCs and hdMSCs were isolated and cultivated by tissue digested-adherent-passage in low-serum medium and the changes of hydroxyproline(Hyp) and TCF-β1 were determined in co-culture system of mdMSCs or hdMSCs and hsFb on day 1 and 4 by samples basic hydrolysis and ELISA respectively. RESULTS: No significant difference was found in the contents of Hyp between groups of different MSCs(F = 0.174) or between groups of different cell density (F = 1.304). The contents of Hyp were significantly different between day 1 and day 4 (F = 8.712, P ＜ 0.01). The level of TGF-β1 was not significantly different between groups of different MSCs(F = 0.062) or between groups of different cell density (F = 1.991). The level of TGF-β1 was significantly different between day 1 and day 4 (F = 0.699, P ＜ 0.05). CONCLUSION: Dermal MSCs of different origin (mouse or human) would not affect the levels of collagen and TGF-β1 when cultivated with hsFb. It is reliable for heterogeneously dermis-derived MSCs to promote tissue repair on hsFb.
NETL
NSTL
万方数据
维普
