Detection of methylation of Caveolin-1 gene exon 2 in gastric carcinoma
AIM: To detect methylation of 5'CpG island in the promoter region of Caveolin-1 gene exon-2 in gastric carcinoma by methylation-specific PCR( MSP) technique and to analyze the role of methylation of Caveolin-1 gene in the genesis and development of gastric carcinoma. METHODS: Thirty samples of gastric carcinoma tissues and 6 samples of normal gastric tissues taken at least 5 cm away from tumors were collected. DNA of the tissues was extracted by standard proteinase K digestion and phenol/ chloroform extraction protocols. After the treatment with CpGenome DNA Modification Kit, the extracted DNA was amplified using primers either methylated or unmethylated. The PCR products were analyzed by both agarose gel electrophoresis and sequencing. RESULTS: No methylalion of the 5'CpG island in the promoter region of Caveolin-1 gene exon-2 was observed in the 6 samples of normal gastric tissues. In the 30 samples of gastric carcinoma tissues, the methylation positive rate of 5'CpG island in the promoter region of Caveolin-1 gene exon-2 was 90% (27/30), among which 16 cases (53.3%) were only amplified by methylated primers and 11 cases(36.7%) were amplified both by methylated and unmethylated primers. The methylation rate of the promoter region of Caveolin-1 gene exon-2 in gastric carcinoma was significantly higher than that in normal gastric tissues. CONCLUSION: The methylation of the 5'CpG island in the promoter region of Caveolin-1 gene exists in gastric carcinoma and it may be involved in the early process of gastric carcinogenesis.
NETL
NSTL
万方数据
维普
