肺硬化性血管瘤整个病变的克隆性
Clonality of whole lesion of pulmonary sclerosing haemangioma
任凯夕 1巩丽 2张丽 2李艳红 3张伟 2朱少君 2韩秀娟 2姚丽 2兰淼2
作者信息
- 1. 第四军医大学口腔医学系临床医学2005级五队,陕西西安710033
- 2. 唐都医院病理科,陕西西安710038
- 3. 唐都医院妇产科,陕西西安710038
- 折叠
摘要
7例31.8%(7/22)显示PGK基因位点多态性.经Hpa Ⅱ酶切后,所有具有多态性的标本2个条带中有1个条带的密度明显减弱或消失,而另1个条带保留,证明其均为肿瘤性增生.结论:PSH是一种真性肿瘤,构成PSH的2种主要细胞起源于同一类型细胞.
Abstract
AIM: To investigate the clonal status of the whole lesion of pulmonary sclerosing haemangioma(PSH). METHODS: PSH whole lesions composed of polygonal cells and surface cuboidal cells, and the surrounding normal lung tissues were microdissected from routine hematoxylin and eosin-stained sections, respectively in 22 female patients. Genomic DNA was extracted, pretreated with methylation-sensitive restrictive endonuclease Hpa Ⅱ, and then amplified via nested polymerase chain reaction (PCR) for phosphoglycerate kinase gene. Clonal analysis was conducted based on X-chromosomal inactivation mosaicism and polymorphism at PGK loci in female somatic cells and the clonality of the PSH whole lesion was observed. RESULTS: Microscopically, typical histopathological characteristics, including solid, papillary, sclerotic and hemorrhagic patterns, were observed in all the 22 cases. The lesions were composed of round or polygonal cells and surface cuboidal cells. Clonality assay showed that the rate of ploymorphism was 31.8% (7/22) for PGK gene. Following digestion by Hpa Ⅱ, one of the two bands in all the samples disappeared, while the other band reserved, suggesting their neoplastic nature. CONCLUSION: The PSH whole lesion is a real neoplastic lesion. Its two kinds of cells originate from a common type of cell.
关键词
单克隆/硬化性血管瘤/肺肿瘤/磷酸甘油酸激酶Key words
monoclonal/sclerosing haemangioma/lung neoplasms/phosphoglycerate kinase引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据