Clonality of whole lesion of pulmonary sclerosing haemangioma
AIM: To investigate the clonal status of the whole lesion of pulmonary sclerosing haemangioma(PSH). METHODS: PSH whole lesions composed of polygonal cells and surface cuboidal cells, and the surrounding normal lung tissues were microdissected from routine hematoxylin and eosin-stained sections, respectively in 22 female patients. Genomic DNA was extracted, pretreated with methylation-sensitive restrictive endonuclease Hpa Ⅱ, and then amplified via nested polymerase chain reaction (PCR) for phosphoglycerate kinase gene. Clonal analysis was conducted based on X-chromosomal inactivation mosaicism and polymorphism at PGK loci in female somatic cells and the clonality of the PSH whole lesion was observed. RESULTS: Microscopically, typical histopathological characteristics, including solid, papillary, sclerotic and hemorrhagic patterns, were observed in all the 22 cases. The lesions were composed of round or polygonal cells and surface cuboidal cells. Clonality assay showed that the rate of ploymorphism was 31.8% (7/22) for PGK gene. Following digestion by Hpa Ⅱ, one of the two bands in all the samples disappeared, while the other band reserved, suggesting their neoplastic nature. CONCLUSION: The PSH whole lesion is a real neoplastic lesion. Its two kinds of cells originate from a common type of cell.
NETL
NSTL
万方数据
维普
