鲍曼不动杆菌对碳青霉烯类抗生素耐药基因研究
Study on gene for resistance of carbap-enems in Acinetobacter baumannii
张永明 1薛晓东 2魏莲花 3邹风梅 3刘刚 3刘天祥1
作者信息
- 1. 兰州大学第一临床医学院,甘肃兰州730000
- 2. 甘肃省人民医院烧伤整形科,甘肃兰州730000
- 3. 甘肃省检验中心,甘肃兰州730000
- 折叠
摘要
目的:检测耐碳青霉烯类抗菌药物鲍曼不动杆菌携带的耐药基因.方法:K-B琼脂扩散法做抗菌药物敏感实验,聚合酶链式反应技术(PCR)在所收集菌株中扩增BL4imp,BL4 vim,OXA-24,OXA-23基因.结果:筛选出亚胺培南耐药的鲍曼不动杆菌(IMRABs)12株,采用PCR技术在3株IMRABs同时扩增出BLA vim,OXA-23基因;7株扩增出OXA-23基因;2株耐药基因丢失.结论:部分表型为产金属酶的IMRABs耐药机制不单纯与携带金属酶基因相关,而是与携带其他耐药基因协同作用有关.
Abstract
AIM: To study the gene for resistance of carbapenems in Acinetobacter baumannii. METHODS: Drug susceptibility was determined by K-B and the fragment of BLA vim, BLA imp, OXA-23 and OXA-24 genes were amplified by polymerase chain reactions. RESULTS: In the 12 strains of lipenem-resistant Acinetobacter baumannii, the fragment of BLA vim, OXA-23 genes was amplified simultaneously from 3 strains and the fragment of OXA-23 gene was amplified from 7 strains. The gene coding drug resistance was lost in the other 2 strains. CONCLUSION: Some Iipenem-resistant Acinetobacter baumannii which produce metallo-β-lactamase carry not only BAL vim gene coding metallo-β-lactamase but also some other drug resistant genes.
关键词
鲍曼不动杆菌/金属酶/OXA-23基因Key words
Acinetobacter baumannii/metallo-β-lactamase/OXA-23 gene引用本文复制引用
基金项目
甘肃省科技支撑计划(0708NKCA096)
甘肃省社会发展计划(0708NKCA096)
出版年
2009
NETL
NSTL
维普
万方数据