小檗碱对兔红细胞膜流动性及膜蛋白荧光的影响
Effect of berberine on membrane fluidity and membrane protein fluorescence of rabbit red-blood cells
杨勇 1叶小利 2李学刚3
作者信息
- 1. 怀化医学高等专科学校药学系,湖南怀化418000
- 2. 西南大学生命科学学院,重庆400716
- 3. 西南大学药学院,重庆400716
- 折叠
Abstract
AIM: To investigate the effects of berberine on the membrane fluidity and membrane protein fluorescence of red-blood cell membrane. METHODS: With rabbit red-blood cell membrane as the experimental material and DPH (1,6-diphenyl-1, 3, 5-hexatriene)as the fluorescence probe, the effect of berberine on the cell membrane fluidity and membrane protein fluorescence was examined by fluorescence spectrum analytical method. RESULTS: The P value of rabbit red-blood cell membrane fluidity was decreased and the fluorescence of rabbit red-blood cell membrane protein was quenched by berberine dose-dependently. CONCLUSION: Berberine effectively enhances the membrane fluidity, suggesting that the increasing membrane fluidity is related to the multiple cardiovascular pharmacological activities. The effect of berberine on the membrane protein may result from the changes of membrane fluidity.
关键词
小檗碱/药理学/红细胞膜/膜蛋白类/药物生理作用/抗菌作用Key words
berberine/pharmacology/erythrocyte membrane/membrane proteins/physiological effects of drugs/antimicrobial activity引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据