Effects of emodin on K562 leukemia cells apoptosis and Caspase-3 expression in vitro
AIM:To study the mechanism of K562 leukemia cell proliferation and apoptosis induced by emodin. METHODS: Inhibitory effect of emodin on the proliferation of K562 leukemia cells was assayed by MTT method. The morphologic changes of K562 cells were detected by electronmicroscope and apoptosis was observed by TUNEL. The changes of cell cycle and apoptosis were detected by flow cytometry and the activity of Caspase-3 was examined by chromatometry to analyze apoptosis of K562 cells. The transcription level of Caspase-3 was determined by RT-PCR. RESULTS:The proliferation of K562 leukemia cells was significantly inhibited by emodin. The IC50 of K562 cells inhibited by emodin for 24 and 72 h was respectively 80 and 40 μmol/L. Typical morphological changes of K562 cells were observed and emodin induced K562 cells toward apoptosis. The result of flow cytometry indicated that the cell cycle was blocked in G0/G1 phase (P ＜ 0.01). After treated with emodin, the activity of Caspase-3 was significant higher than that of normal control (P ＜ 0.01). RT-PCR showed that the expression of Caspase-3 mRNA in cells increased. CONCLUSION: Emodin inhibits the proliferation and induces apoptosis of K562 leukemia cells, possibly by up-regulating the expression of Caspase-3.
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