Effect of carthamus tinctorius on connective tissue growth factor expression in hypertrophic scar of rabbit's ears
AIM: To investigate the effect of carthamus tinctorius on connective tissue growth factor (CTGF) expression in hypertrophic scars (HS) of rabbit's ears. METHODS: Twenty-seven New Zealand rabbits were chosen as experiment animals and the models of HS were established with 2 HS in each ear. On the 45th day after the model establishment, HS were injected with nothing, sodium chloride, carthamus tinctorius (125 g/L) and carthamus tinctorius (500 g/L), respectively once a week for 4 weeks. At the 2nd, 4th and 6th week after injection, HS and skin of 8 rabbits were cut and detected by immunohistochemical method (sp) and reverse transcription polymerase chain reaction (RTPCR). RESULTS: The expressions of CTGF protein and CTGF-mRNA in HS with carthamus tinctorius(125 g/L) and earthamus tinctorius (500 g/L) were lower than those in positive control group and sodium chloride group at the 2nd, 4th and 6th week after injection(P ＜ 0.05). The expressions of CTGF protein and CTGF-mRNA in HS with carthamus tinctorius(500 g/L) were the lowest (P ＜ 0.05). CONCLUSION: Carthamus tinctorius (125 g/L) and carthamus tinctorius(500 g/L)inhibit the expression of CTGF in hypertrophic scar of rabbit's ears. The effect of carthamus tinctnrius(500 g/L)is stronger than carthamus tinctorius(125 g/L).
NETL
NSTL
万方数据
维普
