Hypermethylation of CpG island at p16 gene promoter in gastric carcinomas
AIM: To investigate the expression of hypermethylation of CpG island at the pl6 gene promoter in gastric carcinomas and its significance. METHODS: Hypermethylation of CpG island at the p16 gene promoter was determined by methylation-specific polymerase chain reaction (MSP) analysis. RESULTS: CpG-island hypermethylaion was not detected in control biopsy specimens but was identified in human gastric cancer cell lines and 74.1% (23/31) of gastric carcinoma patients. CpG-island hypermethylaion of p16 gene was detected in 17 adjacent gastric tissues (73.9%, 17/23). In primary gastric tumors, no significant association was found between tumor differentiation status and lymph node metastasis and CpG island methylation status of p16 gene (P＞0.05, respectively). CONCLUSION: Hypermethylation of CpG island at the p16 gene promoter is detected commonly in gastric carcinoma. p16 promoter hypermethylation may be involved in the early gastric carcinogenesis process.
NETL
NSTL
万方数据
维普
