Regulatory effect of dendritic cells transfected with TGF-β1 gene on secretion of IFN-γ and NO of T lymphocytic cells in rats with experimental autoimmune myasthenia gravis
AIM: To explore the regulatory effect of dendritic cells (DC) transfected with TGF-β1 gene on the secretion of IFN-γ and NO of T lymphocytic cells in the Lewis rats with experimental autoimmune myasthenia gravis (EAMG). METHODS: Twenty-five healthy female Lewis rats were divided randomly into 5 groups: normal group, EAMC group, DC treatment group, pcDNA3-TGFβ1-DC treatment group and normal saline group. All the rate except those in normal group were immunized with acetyl-choline receptor (AChR) protein extracted from electric organ of Narcine timilei and completed Freund's adjuvant (CFA).2 × 106 pcDNA3-TGFβ1-DCs/rat were injected subcutaneously into the backs of the rats immunized 5 d earlier with AChR + CFA. The rats in DC treatment group and normal saline group were injected with untreated DCs and normal saline respectively. Seven weeks after the first immunization, T lymphocytic cells in every rat were separated from the spleen. After a 48-h culture, the content of IFN-γ in the supernatant was detected by ELISA and the level of NO on the T lymphocytic cells was examined using chemistry method. RESULTS: Low content of IFN-γ in the supernatant was found in the normal rats and the content increased markedly in EAMG rats (P ＜ 0.01). No significant difference was observed in the contents of IFN-γ between EAMG group and pcDNA3-TGF-β1-DC group. The level of NO was low in normal rats but increased significantly in EAMG rats (P ＜ 0.001). The level in pcDNA3-TGF-β1-DC group was higher than that in EAMG group (P ＜ 0.05), but with no statistically difference between EAMG group, DC treatment group and normal saline group. CONCLUSION:The regulation of the excretion of IFN-γ and NO on T lymphocytic cells may play a critical role in the treatment with DCs transfected with pcDNA3-TGFβ1 in EAMG rats.
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万方数据
维普
