The influence of fetal microchemisrism to dendritic cells in the thyroid glangs
AIM: To analyze the relationship between fetal microchemisrism and dendritic cells (DCs) in the thyroid tissue to explain the influence of the fetal microchemirism to Graves' disease(GD). METHODS: Saventy-two mice were injected with Ad-TSH289 (5 × 108 IU) and mate with male mice until 2 weeks of pregnancy or 2 weeks post-partum. SRY gene was tested by real-time PCR. S-100 protein and CD80 were used as the special marker of the DCs and mature DCs respectively. These markers were stained in the thyroid tissue of GD animal model by HC. RESULTS: The number of the S-100 positive cells in the thyroid tissue of pregnancy and post-partum GD animal model group was more than that of the simple immunized GD animal models (P ＜ 0.05). But the number of CD80 positive cells of post-partum group was the most among those 3 groups (P ＜ 0.05). There were statistically positive co-relationship between relative expression level of SRY gene and number of dendritic cells (S-100 positive cells) in the postpartum GD models (r = 0.5648, P ＜ 0.05). At the same time, there were statistically positive co-relationship between level of SRY gene and mature dendritic cells (CD80 positive cells) in the pregnancy and post-partum GD models (r = 0.4262, P ＜ 0.05). CONCLUSION: Intrathyroidal fetal microchimerism may play a very important role to modulate the autoimmune response for post-partum GD animal models by influencing the mature of dendritic cells in the thyroid glands.
NETL
NSTL
万方数据
维普
