Significance of the matrix metalloproteinase 9 and tissue inhibitor metalloproteinase 1 in lung tissues, BALF and serum of asthma rats
AIM: To explore the roles of matrix metalloproteinase 9 (MMP-9) and tissue inhibitor metalloproteinase 1 (TIMP-1) in the morbidity of asthma and their relation with airway remodeling. METHODS: Twenty young Wistar rats were randomly divided into normal control group and asthma model group, with 10 in each group. Ovalbumin (OVA) was adopted for sensitizing asthma rat model. After 14 d, 1% ovalbumin solution aerosol was given to rats of asthma model group, which had been excited for 20 min, once daily for 6 weeks. Aerosol inhalation with normal saline was adopted for control group. Asthmatic attack symptoms, airway wall thickness, thickness of smooth muscle and collagen deposition were observed in each group. MMP-9 and TIMP-1 levels in serum and BALF of experimental rats were detected by ELBA and their relationship with MMP-9 and TIMP-1 expression in lung tissues was analyzed. RESULTS: MMP-9 and TIMP-1 expression was reduced significantly in lung tissues of asthma model and their airway walls and smooth muscle thickness were significantly higher than those in control group. The serum and BALF MMP-9 and TIMP-1 levels were significantly higher in asthma model than those in normal control group, with statistically significant difference. MMP-9 and TIMP-1 levels in serum and BALF were positively correlated with airway wall thickness, smooth muscle thickness and collagen area, and negatively correlated with MMP-9 and TIMP-1 expression in lung tissues. CONCLUSION: MMP-9 and TIMP-1 are involved in the airway remodeling process. MMP-9 and TIMP-1 levels in serum and BALF are of some significance for clinical diagnosis.
asthmaairway remodelinggelatinase Btissue inhibitor of metalloproteinase 1
NETL
NSTL
万方数据
维普
